Przybylska-Gornowicz B, Lewczuk B, Møller M
Department of Histology and Embryology, Faculty of Veterinary Medicine, Warmia-Masurian University, Olsztyn, Poland.
Folia Histochem Cytobiol. 2000;38(2):85-90.
An immunohistochemical study of the pig pineal gland was carried out using polyclonal rabbit antiserum raised against substance P (SP). The pineal glands were taken from the newborn, 21-day- and 7-month-old female pigs. Immunoreactive nerve fibers were observed in the pineal gland as well as in the posterior commissure and habenular areas. The bundles of SP-immunoreactive fibers were also seen in the subependymal layer of the pineal tissue. The single SP-immunoreactive nerve fibers and few small bundles of nerve fibers were located with equal density throughout the pineal gland, in the connective tissue septa and in the parenchyma. SP-immunoreactive cell bodies were observed in the medial habenular nucleus. The obtained results point to this nucleus as one of the central sources of SP innervation in the pig pineal gland. The study did not show any differences in the distribution and the density of SP-immunoreactive nerve fibers between newborn, 21-day- and 7 month-old pigs.
利用抗P物质（SP）的兔多克隆抗血清对猪松果体进行了免疫组织化学研究。松果体取自新生、21日龄和7月龄的雌性猪。在松果体以及后连合和缰核区域观察到免疫反应性神经纤维。在松果体组织的室管膜下层也可见到SP免疫反应性纤维束。单个SP免疫反应性神经纤维和少量小神经纤维束在整个松果体、结缔组织间隔和实质中密度相等。在内侧缰核中观察到SP免疫反应性细胞体。所得结果表明该核是猪松果体中SP神经支配的主要中枢来源之一。该研究未显示新生、21日龄和7月龄猪之间SP免疫反应性神经纤维的分布和密度有任何差异。
