Cazón Narvaez A V, Sühring S S
Facultad de Ciencias Naturales, Universidad Nacional de Salta, Buenos Aires, Salta, Argentina.
Rev Biol Trop. 1999 Mar-Jun;47(1-2):245-9.
Fecal bile acid patterns have been used successfully to identify scats. Neotropical felid scats are capable of this biochemical identification because they present low concentrations of plant pigments that would interfere in fecal bile acids detection. However, neotropical felid scats have poor quantities of bile acids, so we developed in this work a proper technique for their extraction, visualization and determination. Twenty eighth feces of seven different felid species, collected from Zoological and Wildlife Parks, were dried and pulverized. The procedure for analyzing feces is: Take one g of pulverized feces and shake for 3 hr at room temperature in 20 ml benzene:methanol; filter and evaporate to 5 ml. Spot on TLC plate and develop in toluene:acetic acid:water. Dry and visualize with anisaldehyde. Field collected scats could be identified by the bile acids pattern revealed by this specific technique and, then, used as a source of information for distribution, density and food habits studies.
粪便胆汁酸模式已成功用于识别粪便。新热带猫科动物的粪便能够进行这种生化识别，因为它们所含植物色素浓度低，不会干扰粪便胆汁酸的检测。然而，新热带猫科动物的粪便中胆汁酸含量较少，因此我们在这项研究中开发了一种合适的提取、可视化和测定技术。从动物园和野生动物园收集了七种不同猫科动物的28份粪便，进行干燥和粉碎。分析粪便的步骤如下：取1克粉碎后的粪便，在室温下与20毫升苯：甲醇混合振荡3小时；过滤并蒸发至5毫升。点样于薄层层析板上，用甲苯：乙酸：水展开。干燥后用茴香醛显色。通过这种特定技术揭示的胆汁酸模式可以识别野外收集的粪便，进而将其用作分布、密度和食性研究的信息来源。
