Buch B, Papert A I, Shear M
J Oral Pathol. 1979 Apr;8(2):94-102. doi: 10.1111/j.1600-0714.1979.tb01628.x.
Histological changes were studied in the rat tongue for varying periods of time up to 14 days following the application of a cryoprobe to the dorsal surface of rat tongue. Within minutes of the application, focal subepithelial vesiculation and hyperemia occurred. At 3 h there was evidence of damage to capillary walls, resulting in hemorrhage. Arterioles and venules were thrombosed and there were degenerative changes in the muscle layer of their walls. At 6 h ventral epithelium showed evidence of damage. At this stage vessel walls were pavemented with neutrophilic polymorphonuclear leukocytes (PMN) and some of the latter were seen in the vessel walls. At 24 h abundant PMN, many of them necrotic, were present in the vessel walls, apparently unable to pass through them. At 48 h numerous PMN were seen adhering to degenerating muscle fibers. By 4 days necrotic changes extended through the full thickness of the tongue, but concurrently it was observed that the larger thrombosed vessels were becoming recanalized. At 5 days repair by granulation tissue was taking place and at 6 days the vasculature was virtually reconstituted. By 14 days healing was complete.
在将冷冻探头应用于大鼠舌背表面后的长达14天的不同时间段内，对大鼠舌头的组织学变化进行了研究。在应用后的几分钟内，出现了局灶性上皮下小泡形成和充血。3小时时，有毛细血管壁受损的证据，导致出血。小动脉和小静脉血栓形成，其管壁肌层出现退行性变化。6小时时，腹侧上皮显示出受损迹象。在此阶段，血管壁被嗜中性多形核白细胞（PMN）覆盖，其中一些可见于血管壁内。24小时时，大量PMN（其中许多已坏死）存在于血管壁内，显然无法穿过血管壁。48小时时，可见大量PMN附着于变性的肌纤维上。到第4天，坏死变化扩展至舌头全层，但同时观察到较大的血栓形成血管开始再通。第5天，肉芽组织开始修复，第6天，脉管系统基本重建。到第14天，愈合完成。
