Katayama H, Ishihama Y, Asakawa N
Department of Analytical Chemistry, Eisai Co., Ltd., Tsukuba, Ibaraki, Japan.
J Chromatogr A. 2000 Apr 14;875(1-2):315-22. doi: 10.1016/s0021-9673(99)01347-3.
Perfect control of electroosmotic flow (EOF) was achieved by dovetailing successive multiple ionic-polymer layer (SMIL) coated capillaries. The direction and magnitude of the EOF was perfectly controllable over the pH range 2-13. Zone diffusion was not observed, even if the inner wall of the dovetailed capillary was discontinuous, or if the sample zone passed through the connected part of the capillary because the RSDs of migration time, theoretical plates, symmetry factor and S/N of the marker were almost the same when seamless capillary and dovetailed capillary were compared. The dovetailed capillary was applied to cyclodextrin modified capillary zone electrophoresis. The control of the EOF enabled us to control both the resolution and the migration order of the enantiomers. The migration time was also controllable and, therefore, the best condition between separation and migration time could be determined by controlling the EOF. Partial filling affinity electrokinetic chromatography with a protein used as a chiral selector was also studied. The migration of the pseudostationary phase was controllable by EOF, and detection of the solute at 214 nm was possible. Therefore, the EOF-controlled dovetailed capillary has great potential to expand the application of the separation technique.
通过对接连续多层离子聚合物层（SMIL）涂层毛细管实现了对电渗流（EOF）的完美控制。在pH值2至13的范围内，EOF的方向和大小均可完美控制。即使对接毛细管的内壁不连续，或者样品区穿过毛细管的连接部分，也未观察到区带扩散，因为在比较无缝毛细管和对接毛细管时，迁移时间、理论塔板数、对称因子和标记物的信噪比的相对标准偏差（RSD）几乎相同。对接毛细管应用于环糊精修饰的毛细管区带电泳。对EOF的控制使我们能够控制对映体的分离度和迁移顺序。迁移时间也是可控的，因此，通过控制EOF可以确定分离和迁移时间之间的最佳条件。还研究了以蛋白质作为手性选择剂的部分填充亲和电动色谱法。假固定相的迁移可通过EOF控制，并且可以在214nm处检测溶质。因此，EOF控制的对接毛细管在扩展分离技术的应用方面具有巨大潜力。
