Piette V, Fillet M, Lindner W, Crommen J
Scientific Institute of Public Health--Louis Pasteur, Bruxelle, Belgium.
J Chromatogr A. 2000 Apr 14;875(1-2):353-60. doi: 10.1016/s0021-9673(00)00083-2.
A non-aqueous capillary electrophoretic method developed with quinine and tert.-butyl carbamoylated quinine as chiral selectors for the enantioseparation of N-protected amino acids was applied to the investigation of other quinine derivatives as chiral additives. The optimum composition of the background electrolyte was found to be 12.5 mM ammonia, 100 mM octanoic acid and 10 mM chiral selector in an ethanol-methanol (60:40, v/v) mixture. Under these conditions, a series of chiral acids, as various benzoyl, 3,5-dinitrobenzoyl and 3,5-dinitrobenzyloxycarbonyl amino acid derivatives were investigated with regards to selectand-selector relationships and enantioselectivity employing quinine, quinidine, cinchonine, cinchonidine, tert.-butyl carbamoylated quinine, tert.-butyl carbamoylated quinidine, dinitrophenyl carbamoylated quinine and cyclohexyl carbamoylated quinine as chiral selector.
一种以奎宁和叔丁基氨基甲酰化奎宁作为手性选择剂用于N-保护氨基酸对映体拆分的非水毛细管电泳方法，被应用于研究其他奎宁衍生物作为手性添加剂的情况。发现背景电解质的最佳组成是在乙醇-甲醇（60:40，v/v）混合物中含有12.5 mM氨、100 mM辛酸和10 mM手性选择剂。在这些条件下，使用奎宁、奎尼丁、辛可宁、辛可尼丁、叔丁基氨基甲酰化奎宁、叔丁基氨基甲酰化奎尼丁、二硝基苯基氨基甲酰化奎宁和环己基氨基甲酰化奎宁作为手性选择剂，研究了一系列手性酸，如各种苯甲酰基、3,5-二硝基苯甲酰基和3,5-二硝基苄氧基羰基氨基酸衍生物的选择物-选择剂关系和对映选择性。
