de Girolamo P, Arcamone N, Lucini C, Castaldo L, Vega J A, Gargiulo G
Università di Napoli Federico II, Dipartimento di Strutture, Funzioni e Tecnologie Biologiche, Italy.
Anat Embryol (Berl). 2000 May;201(5):429-33. doi: 10.1007/s004290050330.
Neurotrophins are involved in the development and maintenance of some neuronal populations, acting through tyrosine protein kinase (Trk) receptors, TrkA, TrkB and TrkC. In addition, recent studies have demonstrated the occurrence of Trk receptors in a wide variety of adult and embryonic non-neuronal tissues in vertebrates, including kidney. Nevertheless no data are available on Trk proteins distribution in teleost kidney. The present study, by using an immunoperoxidase technique, analyses Trk receptor protein distribution in the kidney of three species of freshwater and saltwater teleost. TrkA-like immunoreactivity was the only one detected in all examined species, while TrkB-like immunoreactivity was completely absent and TrkC-like immunoreactivity was detected only in goldfish. The TrkA immunoreactive cells were mainly localised in the collecting duct system, as this system was the only one to also display TrkC. Such data could provide new clues to Trk in fish and aid assessment of the role of Trk protein receptors during vertebrate evolution.
神经营养因子通过酪氨酸蛋白激酶（Trk）受体TrkA、TrkB和TrkC参与某些神经元群体的发育和维持。此外，最近的研究表明，Trk受体存在于包括肾脏在内的脊椎动物多种成年和胚胎非神经元组织中。然而，关于硬骨鱼肾脏中Trk蛋白的分布尚无数据。本研究采用免疫过氧化物酶技术，分析了三种淡水和咸水硬骨鱼肾脏中Trk受体蛋白的分布。在所有检测的物种中，仅检测到TrkA样免疫反应性，而完全未检测到TrkB样免疫反应性，仅在金鱼中检测到TrkC样免疫反应性。TrkA免疫反应性细胞主要定位于集合管系统，因为该系统是唯一同时显示TrkC的系统。这些数据可为鱼类中的Trk提供新线索，并有助于评估Trk蛋白受体在脊椎动物进化过程中的作用。
