Ni Y H, Lin J T, Huang S F, Yang J C, Chang M H
Departments of Pediatrics, Internal Medicine, and Pathology, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei.
J Pediatr. 2000 Jun;136(6):823-7.
Invasive and noninvasive tests have been developed for the diagnosis of Helicobacter pylori infection. Because H pylori infection is acquired in childhood and adolescence, accurate diagnosis of the infection in the pediatric population is important. We conducted a study to compare invasive tests: culture, biopsy urease test, histology, and polymerase chain reaction on gastric biopsy specimens, with noninvasive tests: serology, (13)C-urea breath test, and a new diagnostic modality, stool antigen test to diagnose H pylori infection. A total of 53 children with symptoms were enrolled in this study, and all had completed the 7 diagnostic tests for H pylori. All the diagnostic tests except serology were excellent methods of diagnosing H pylori infection in children; the diagnostic accuracy was as follows: stool antigen test 96.2%, biopsy urease test 96.2%, histology 98.1%, polymerase chain reaction 94.3%, culture 98.1%, (13)C-urea breath test 100%, and serology 84.9%. The stool antigen test, being highly sensitive and specific, will be potentially very helpful in diagnosing H pylori infection in children.
已开发出侵入性和非侵入性检测方法用于诊断幽门螺杆菌感染。由于幽门螺杆菌感染在儿童期和青春期获得，因此准确诊断儿科人群中的感染很重要。我们进行了一项研究，比较侵入性检测方法：对胃活检标本进行培养、活检尿素酶试验、组织学检查和聚合酶链反应，与非侵入性检测方法：血清学、（13）C-尿素呼气试验，以及一种新的诊断方法，即粪便抗原检测来诊断幽门螺杆菌感染。本研究共纳入53名有症状的儿童，所有儿童均完成了7项幽门螺杆菌诊断检测。除血清学外，所有诊断检测都是诊断儿童幽门螺杆菌感染的优秀方法；诊断准确性如下：粪便抗原检测96.2%，活检尿素酶试验96.2%，组织学检查98.1%，聚合酶链反应94.3%，培养98.1%，（13）C-尿素呼气试验100%，血清学84.9%。粪便抗原检测具有高度敏感性和特异性，在诊断儿童幽门螺杆菌感染方面可能非常有用。
