Chollet P, Chassagne J, Guérin D, Sauvezie B, Bidet J M, Bétail G, Plagne R
Rev Can Biol. 1976 Mar;35(1):1-9.
Cell electrophoresis enables the separation of the lymphocytes in normal human blood into two principal groups, as a function of their migration speed in relation to 1 mum.sec -1..v-1.cm. In 42 healthy adults, 19,9 % of the lymphocytes have a slower migration, and 80,1 % a faster migration than the reference speed. Two known methods are used for the selection of the lymphocytic populations : spontaneous rosetting with sheep red blood cells, a property of the T lymphocytes, and the adherence to nylon wool columns, which is dominant in the case of B lymphocytes. The cells which do not form spontaneous rosettes, and the cells adhering to nylon wool columns have above all a slow migration. On the contrary, cells which do not adhere to nylon columns have a fast migration. These arguments are in favour of the T nature of the rapid migrating lymphocytes, and of the B nature of the slow migrating lymphocytes.
细胞电泳能够根据淋巴细胞相对于1μm·秒⁻¹·伏⁻¹·厘米的迁移速度,将正常人血液中的淋巴细胞分为两个主要组。在42名健康成年人中,19.9%的淋巴细胞迁移速度较慢,80.1%的淋巴细胞迁移速度比参考速度快。有两种已知方法用于选择淋巴细胞群体:利用T淋巴细胞的特性与绵羊红细胞进行自发凝集,以及在B淋巴细胞中占主导的对尼龙毛柱的黏附。不形成自发凝集的细胞以及黏附于尼龙毛柱的细胞主要具有缓慢的迁移速度。相反,不黏附于尼龙柱的细胞具有快速的迁移速度。这些论据支持快速迁移淋巴细胞的T细胞性质以及缓慢迁移淋巴细胞的B细胞性质。