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[通过尼龙柱和自发玫瑰花结纯化的人淋巴细胞的电泳迁移率]

[Electrophoretic mobility of human lymphocytes purified by nylon columns and spontaneous rosettes].

作者信息

Chollet P, Chassagne J, Guérin D, Sauvezie B, Bidet J M, Bétail G, Plagne R

出版信息

Rev Can Biol. 1976 Mar;35(1):1-9.

PMID:1084002
Abstract

Cell electrophoresis enables the separation of the lymphocytes in normal human blood into two principal groups, as a function of their migration speed in relation to 1 mum.sec -1..v-1.cm. In 42 healthy adults, 19,9 % of the lymphocytes have a slower migration, and 80,1 % a faster migration than the reference speed. Two known methods are used for the selection of the lymphocytic populations : spontaneous rosetting with sheep red blood cells, a property of the T lymphocytes, and the adherence to nylon wool columns, which is dominant in the case of B lymphocytes. The cells which do not form spontaneous rosettes, and the cells adhering to nylon wool columns have above all a slow migration. On the contrary, cells which do not adhere to nylon columns have a fast migration. These arguments are in favour of the T nature of the rapid migrating lymphocytes, and of the B nature of the slow migrating lymphocytes.

摘要

细胞电泳能够根据淋巴细胞相对于1μm·秒⁻¹·伏⁻¹·厘米的迁移速度,将正常人血液中的淋巴细胞分为两个主要组。在42名健康成年人中,19.9%的淋巴细胞迁移速度较慢,80.1%的淋巴细胞迁移速度比参考速度快。有两种已知方法用于选择淋巴细胞群体:利用T淋巴细胞的特性与绵羊红细胞进行自发凝集,以及在B淋巴细胞中占主导的对尼龙毛柱的黏附。不形成自发凝集的细胞以及黏附于尼龙毛柱的细胞主要具有缓慢的迁移速度。相反,不黏附于尼龙柱的细胞具有快速的迁移速度。这些论据支持快速迁移淋巴细胞的T细胞性质以及缓慢迁移淋巴细胞的B细胞性质。

相似文献

1
[Electrophoretic mobility of human lymphocytes purified by nylon columns and spontaneous rosettes].[通过尼龙柱和自发玫瑰花结纯化的人淋巴细胞的电泳迁移率]
Rev Can Biol. 1976 Mar;35(1):1-9.
2
Electrophoretic mobility of human lymphocytes purified by nylon wool columns and spontaneous sheep red blood cells rosetting techniques correlation with immunofluorescence.通过尼龙毛柱和自发绵羊红细胞玫瑰花结技术纯化的人淋巴细胞的电泳迁移率与免疫荧光的相关性
J Immunol Methods. 1976;11(1):25-35. doi: 10.1016/0022-1759(76)90015-6.
3
[Isolation and electrophoretic characteristics of 3 lymphocyte populations of normal human blood].
C R Seances Soc Biol Fil. 1975;169(6):1491-501.
4
Electrophoretic mobility of peripheral non-B human Fc gamma-receptors bearing lymphocytes.携带外周非B人类Fcγ受体的淋巴细胞的电泳迁移率。
Biomedicine. 1980 Feb;32(1):31-41.
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[Application of free flow preparative methods for the separation of blood cells].
Rev Fr Transfus Immunohematol. 1975 Dec;18(4):439-55. doi: 10.1016/s0338-4535(75)80031-6.
6
Identification of T and B cell subpopulations in human peripheral blood: electrophoretic mobility distributions associated with surface marker characteristics.
J Immunol. 1976 Jul;117(1):115-23.
7
Separation of T and B lymphocytes by nylon wool columns: evaluation of efficacy by functional assays in vivo.用尼龙毛柱分离T淋巴细胞和B淋巴细胞:通过体内功能测定评估效果。
J Immunol. 1974 Oct;113(4):1093-7.
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Separation of T and B lymphocytes by nylon fibre columns.用尼龙纤维柱分离T淋巴细胞和B淋巴细胞。
Med Lab Sci. 1981 Mar;38(2):123-5.
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An evaluation of serum sources used in the separation of T- and B-lymphocytes by nylon-wool columns.
Immunol Lett. 1982 May;4(5):285-8. doi: 10.1016/0165-2478(82)90053-0.
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Separation of lymphoid cells on nylon wool columns.
Methods Enzymol. 1984;108:298-302. doi: 10.1016/s0076-6879(84)08095-2.

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