Rutschmann S, Jung A C, Hetru C, Reichhart J M, Hoffmann J A, Ferrandon D
Institut de Biologie Moléculaire et Cellulaire, UPR 9022 du CNRS, Strasbourg, France.
Immunity. 2000 May;12(5):569-80. doi: 10.1016/s1074-7613(00)80208-3.
We have isolated two Drosophila lines that carry point mutations in the gene coding for the NF-KB-like factor DIF. Like mutants of the Toll pathway, Dif mutant flies are susceptible to fungal but not to bacterial infections. Genetic epistasis experiments demonstrate that Dif mediates the Toll-dependent control of the inducibility of the antifungal peptide gene Drosomycin. Strikingly, DIF alone is required for the antifungal response in adults, but is redundant in larvae with Dorsal, another Rel family member. In Drosophila, Dif appears to be dedicated to the antifungal defense elicited by fungi and gram-positive bacteria. We discuss in this light the possibility that NF-KB1/p50 might be required more specifically in the innate immune response against gram-positive bacteria in mammals.
我们分离出了两个果蝇品系，它们在编码类核因子κB（NF-κB）样因子DIF的基因中携带点突变。与Toll信号通路的突变体一样，Dif突变果蝇易受真菌感染，但对细菌感染有抗性。遗传上位性实验表明，Dif介导了Toll依赖性的抗真菌肽基因Drosomycin诱导性的调控。引人注目的是，在成虫中，抗真菌反应仅需要DIF，但在幼虫中，它与另一个Rel家族成员背腹蛋白（Dorsal）功能冗余。在果蝇中，Dif似乎专门负责由真菌和革兰氏阳性菌引发的抗真菌防御。据此，我们讨论了NF-κB1/p50在哺乳动物针对革兰氏阳性菌的固有免疫反应中可能更具特异性需求的可能性。
