Mengerink Y, Peters R, Kerkhoff M, Hellenbrand J, Omloo H, Andrien J, Vestjens M, van der Wal S
MI&Q, DSM Research, Geleen, The Netherlands.
J Chromatogr A. 2000 May 5;878(1):45-55. doi: 10.1016/s0021-9673(00)00277-6.
By separating the first six linear and cyclic oligomers of polyamide-6 on a reversed-phase high-performance liquid chromatographic system after sandwich injection, quantitative determination of these oligomers becomes feasible. Low-wavelength UV detection of the different oligomers and selective post-column reaction detection of the linear oligomers with o-phthalic dicarboxaldehyde (OPA) and 3-mercaptopropionic acid (3-MPA) are discussed. A general methodology for quantification of oligomers in polymers was developed. It is demonstrated that the empirically determined group-equivalent absorption coefficients and quench factors are a convenient way of quantifying linear and cyclic oligomers of nylon-6. The overall long-term performance of the method was studied by monitoring a reference sample and the calibration factors of the linear and cyclic oligomers.
通过在夹心进样后，在反相高效液相色谱系统上分离聚酰胺-6的前六种线性和环状低聚物，这些低聚物的定量测定变得可行。讨论了不同低聚物的低波长紫外检测以及用邻苯二甲酸二醛（OPA）和3-巯基丙酸（3-MPA）对线性低聚物进行选择性柱后反应检测。开发了一种用于聚合物中低聚物定量的通用方法。结果表明，通过经验确定的基团等效吸收系数和猝灭因子是定量尼龙-6线性和环状低聚物的便捷方法。通过监测参考样品以及线性和环状低聚物的校准因子，研究了该方法的整体长期性能。
