Vaz Gomes A, Wahlestedt C
Center for Genomics Research, Karolinska Institutet, Berzelius väg 37, S-171 77, Stockholm, Sweden.
Eur J Pharmacol. 2000 Jun 2;397(2-3):R3-5. doi: 10.1016/s0014-2999(00)00251-x.
Using a systemic and continuous delivery method based on feeding on a particular strain of transformed Escherichia coli to induce double stranded RNA-mediated interference, we targeted the product of the npr-1 gene, a putative Caenorhabditis elegans homologue of a neuropeptide Y receptor, a G-protein coupled receptor. We were able to reproduce the social behaviour observed for the naturally occurring npr-1 mutant when wild type N2 Bristol eggs developed in a lawn of bacteria producing double stranded RNA for npr-1. This facile approach may also be useful when studying the function of other worm G-protein coupled receptors.
通过基于喂食特定转化大肠杆菌菌株的系统且持续的递送方法来诱导双链RNA介导的干扰，我们靶向了npr-1基因的产物，它是一种假定的秀丽隐杆线虫神经肽Y受体（一种G蛋白偶联受体）的同源物。当野生型N2布里斯托尔虫卵在产生npr-1双链RNA的细菌菌苔中发育时，我们能够重现天然存在的npr-1突变体所观察到的社会行为。当研究其他线虫G蛋白偶联受体的功能时，这种简便的方法可能也有用。
