Ratnasooriya W D, Dharmasiri M G, Wadsworth R M
Department of Zoology, University of Colombo, Colombo 3, Sri Lanka.
Int J Androl. 2000 Jun;23(3):187-91. doi: 10.1046/j.1365-2605.2000.00225.x.
The role of nitric oxide (NO) in libido and fertility of male rats was investigated by administration of the NO synthase inhibitor N-nitro-L-arginine methyl ester (L-NAME) (25 or 50 mg/kg/day). L-NAME caused marked reduction of precoital sexual behaviour, and a failure of most rats to mount or ejaculate during the test interval. In most matings that were successful, recovered spermatozoa were present in normal numbers. In mating experiments, L-NAME profoundly reduced the fertility of male rats. In those animals that did succeed in mating, the quantal pregnancy and the number of implants were reduced. After cessation of treatment with L-NAME, the fertility parameters returned close to normal. The inactive stereoisomer, D-NAME, caused none of the above effects when administered to rats. The results suggest that NO is essential for the expression of normal libido and fertility in male rats. It is likely that NO is required both in the male reproductive tract and in the brain.
通过给予一氧化氮合酶抑制剂N-硝基-L-精氨酸甲酯（L-NAME，25或50毫克/千克/天），研究了一氧化氮（NO）在雄性大鼠性欲和生育能力中的作用。L-NAME导致性交前性行为显著减少，并且大多数大鼠在测试期间无法进行骑跨或射精。在大多数成功的交配中，回收的精子数量正常。在交配实验中，L-NAME显著降低了雄性大鼠的生育能力。在那些成功交配的动物中，定量妊娠和着床数量减少。停止使用L-NAME治疗后，生育参数恢复接近正常。无活性的立体异构体D-NAME给予大鼠后未产生上述任何影响。结果表明，NO对于雄性大鼠正常性欲和生育能力的表达至关重要。NO可能在雄性生殖道和大脑中均是必需的。
