Scott C J, Pereira A M, Rawson J A, Simmons D M, Rossmanith W G, Ing N H, Clarke I J
Department of Physiology, Monash University, Clayton, Victoria, Australia.
J Neuroendocrinol. 2000 Jun;12(6):565-75. doi: 10.1046/j.1365-2826.2000.00490.x.
The distribution of progesterone receptors (PR) was mapped in the hypothalamus of the ewe using immunocytochemistry. These results were confirmed using in situ hybridization with a sheep-specific 35S-labelled riboprobe. In addition, the effect of oestrogen on the level of PR mRNA in the hypothalamus was examined in ovariectomized (OVX) ewes following treatment with an oestrogen implant or without treatment. PR immunoreactive (-ir) cells were readily detected in OVX animals. Labelled cells were observed in four main hypothalamic regions: the preoptic area (POA), including the organum vasculosum of the lamina terminalis, periventricular nucleus (PeVN), ventromedial nucleus (VMN) and the arcuate nucleus (ARC) (including the region ventral to the mamillary recess). In addition, lightly stained PR-ir cells were observed in the supraoptic nucleus and a few PR-ir cells were also found in the diagonal band of Broca. No PR-ir cells were found in the brainstem. PR mRNA-containing cells were found in the same hypothalamic regions as the PR-ir cells. Image analysis of emulsion-dipped slides following in situ hybridization indicated that oestrogen treatment increased (P<0.01) the mean number of silver grains/cell and the density of labelled cells in the VMN and ARC but had no effect on the level of PR mRNA expression in the POA or PeN. The distribution of PR-containing cells in the hypothalamus is similar to that described in other species and all cells were located in nuclei that contain large populations of oestrogen receptor-containing cells. These include regions implicated in the regulation of reproductive neuroendocrine function, and reproductive behaviour. Oestrogen and progesterone synergize to inhibit GnRH secretion and the present results suggest that these functions may involve cells of the VMN and ARC, with oestrogen acting to upregulate PR.
采用免疫细胞化学方法，绘制了孕酮受体（PR）在母羊下丘脑的分布图。使用绵羊特异性的35S标记核糖探针进行原位杂交，证实了这些结果。此外，在切除卵巢（OVX）的母羊中，研究了雌激素植入处理或未处理后，雌激素对下丘脑PR mRNA水平的影响。在OVX动物中很容易检测到PR免疫反应性（-ir）细胞。在下丘脑的四个主要区域观察到标记细胞：视前区（POA），包括终板血管器、室周核（PeVN）、腹内侧核（VMN）和弓状核（ARC）（包括乳头体隐窝腹侧区域）。此外，在视上核中观察到轻度染色的PR-ir细胞，在布罗卡斜带中也发现了一些PR-ir细胞。在脑干中未发现PR-ir细胞。与PR-ir细胞一样，在下丘脑相同区域发现了含有PR mRNA的细胞。原位杂交后对乳胶浸渍玻片进行图像分析表明，雌激素处理增加了（P<0.01）VMN和ARC中平均每个细胞的银颗粒数以及标记细胞的密度，但对POA或PeN中PR mRNA表达水平没有影响。下丘脑含PR细胞的分布与其他物种中描述的相似，所有细胞都位于含有大量含雌激素受体细胞的核中。这些区域包括与生殖神经内分泌功能调节和生殖行为有关的区域。雌激素和孕酮协同抑制促性腺激素释放激素（GnRH）分泌，目前的结果表明，这些功能可能涉及VMN和ARC的细胞，雌激素起到上调PR的作用。
