Dong Z W, Zhou S Y, Li Y, Liu R M
Department of Molecular Biology, National Research Institute for Family Planning, Beijing, People's Republic of China.
J Reprod Med. 2000 May;45(5):410-2.
To detect human parvovirus B19 intrauterine infection in pregnancy with the polymerase chain reaction (PCR).
DNA of chorionic villi and amniotic fluid was extracted and the gene of human parvovirus B19 amplified with PCR.
The study analyzed 61 specimens of chorionic villi and 26 specimens of amniotic fluid and found two positive specimens of chorionic villi and 1 positive specimen of amniotic fluid.
The vertical transmission of human parvovirus B19 infection in early pregnancy may be a pathway of intrauterine infection. Chorionic villus sampling in early pregnancy and PCR could be developed as a method of prenatal diagnosis of human parvovirus B19 intrauterine infection.
采用聚合酶链反应（PCR）检测孕期人细小病毒B19宫内感染情况。
提取绒毛膜绒毛和羊水的DNA，并用PCR扩增人细小病毒B19基因。
该研究分析了61份绒毛膜绒毛标本和26份羊水标本，发现2份绒毛膜绒毛标本呈阳性，1份羊水标本呈阳性。
孕早期人细小病毒B19感染的垂直传播可能是宫内感染的一条途径。孕早期绒毛取样和PCR可作为产前诊断人细小病毒B19宫内感染的一种方法。
