Kozhich A T, Chan C C, Gery I, Whitcup S M
National Eye Institute, National Institutes of Health, Bethesda, Maryland, USA.
Invest Ophthalmol Vis Sci. 2000 Jun;41(7):1823-6.
Endotoxin-induced uveitis (EIU) in rats and mice peaks 24 hours after endotoxin injection and is commonly assumed to be a monophasic disease. This study examined intraocular inflammation at later time points to determine whether endotoxin injection can induce recurrent intraocular inflammation in strains of mice with high or moderate levels of susceptibility to EIU.
EIU was elicited in two mouse strains with high (C3H/HeN) and moderate (FVB/N) susceptibility, by means of intraperitoneal injections of Salmonella typhimurium endotoxin. Inflammatory cells in the anterior and posterior segments of the eye were counted by a masked observer on histologic sections of eyes from 1 to 17 days after endotoxin injection.
A bimodal distribution of inflammatory cell infiltration was noted in eyes from C3H/HeN mice. As previously reported, inflammation peaked at 24 hours after endotoxin injection. However, a second, more pronounced peak of intraocular inflammation occurred approximately 5 days after endotoxin injection. FVB/N mice had a single peak of intraocular inflammation 4 days after injection.
Endotoxin injection in C3H/HeN elicits recurrent intraocular inflammation. The previously unrecognized second peak of inflammation is more severe than the initial inflammatory disease. Studies on this second inflammatory peak may be useful in determining the pathogenesis of recurrent uveitis in humans.
大鼠和小鼠内毒素诱导性葡萄膜炎（EIU）在注射内毒素后24小时达到峰值，通常被认为是一种单相疾病。本研究在更晚的时间点检查眼内炎症，以确定内毒素注射是否能在对EIU具有高或中度易感性的小鼠品系中诱发复发性眼内炎症。
通过腹腔注射鼠伤寒沙门氏菌内毒素，在两种具有高易感性（C3H/HeN）和中度易感性（FVB/N）的小鼠品系中诱发EIU。在内毒素注射后1至17天，由一位不知情的观察者对眼组织切片中眼前段和后段的炎性细胞进行计数。
在C3H/HeN小鼠的眼中观察到炎性细胞浸润的双峰分布。如先前报道，炎症在注射内毒素后24小时达到峰值。然而，在注射内毒素后约5天出现了第二个更明显的眼内炎症高峰。FVB/N小鼠在注射后4天出现单一的眼内炎症高峰。
C3H/HeN小鼠注射内毒素可引发复发性眼内炎症。先前未被认识到的第二个炎症高峰比初始炎症疾病更严重。对这第二个炎症高峰的研究可能有助于确定人类复发性葡萄膜炎的发病机制。
