Hinz T, Allam A, Wesch D, Schindler D, Kabelitz D
Department of Immunology, Paul-Ehrlich-Institute, D-63225 Langen, Germany.
Br J Haematol. 2000 Apr;109(1):201-10. doi: 10.1046/j.1365-2141.2000.01962.x.
Transrearrangements between the T-cell receptor (TCR) VgammaI family and JbetaCbeta loci occur at increased frequencies in patients with ataxia telangiectasia (AT). We have used an optimized reverse transcriptase polymerase chain reaction (RT-PCR) approach to investigate the occurrence of TCRVgamma-JbetaCbeta transrearrangements involving the dominantly used Vgamma element in peripheral blood gammadelta T cells, i.e. Vgamma9. We detected in frame transcripts of Vgamma9-JbetaCbeta transrearrangements in 4/16 AT patients and in 3/13 healthy control donors. A panel of monoclonal antibodies (mAb) against all expressed TCRVgamma elements was used to monitor cell-surface expression of transrearranged TCR. A very low proportion (< 1%) of peripheral blood TCRalphabeta cells expressed Vgamma instead of Vbeta elements. For the first time, we have isolated and molecularly characterized alphabeta T cells with a Vgamma9-JbetaCbeta transrearrangement from two AT patients and we have shown that such TCR are functional. We conclude that functional TCR transrearrangements can also involve Vgamma9, the dominant Vgamma element in peripheral blood gammadelta T cells.
在共济失调毛细血管扩张症（AT）患者中，T细胞受体（TCR）VgammaI家族与JbetaCbeta基因座之间的转重排发生频率增加。我们采用优化的逆转录聚合酶链反应（RT-PCR）方法，研究外周血γδT细胞中涉及主要使用的Vgamma元件（即Vgamma9）的TCRVgamma-JbetaCbeta转重排的发生情况。我们在4/16例AT患者和3/13例健康对照供体中检测到Vgamma9-JbetaCbeta转重排的读框内转录本。使用一组针对所有表达的TCRVgamma元件的单克隆抗体（mAb）来监测转重排TCR的细胞表面表达。外周血TCRalphabeta细胞中极低比例（<1%）表达Vgamma而非Vbeta元件。我们首次从两名AT患者中分离并分子鉴定了具有Vgamma9-JbetaCbeta转重排的alphabeta T细胞，并表明此类TCR具有功能。我们得出结论，功能性TCR转重排也可涉及外周血γδT细胞中的主要Vgamma元件Vgamma9。
