Walter R, Schoedon G, Bächli E, Betts D R, Hossle J P, Calandra T, Joller-Jemelka H I, Fehr J, Schaffner A
Department of Medicine, Division of Haematology, University Hospital, Zürich, Switzerland.
Br J Haematol. 2000 May;109(2):396-404. doi: 10.1046/j.1365-2141.2000.02013.x.
Few human monoblastic cell lines have been characterized to date. We have established the SigM5 cell line from a patient with acute monoblastic leukaemia (FAB M5a). Original leukaemic cells had a karyotype of 47,XY,+8, whereas the cell line showed a stemline clone of 81,XX,Y,Y,1,4,6,7,+8,+8,9,10,10,11,13,16,19[cp], with a minor sideline also present. Cytochemical staining was strongly positive with alpha-naphthylbutyrate acetate esterase, particulate positive with Sudan black and weakly positive for myeloperoxidase. Cells were positive for CD13, CD15, CD18, CD23, CD33, CD38, CD45, CD68 and myeloperoxidase. CD14 expression was 3-15%. SigM5 constitutively secreted interleukin (IL)-2, IL-8, IL-10, tumour necrosis factor (TNF)-alpha, ferritin, lysozyme, N-elastase and neopterin upon stimulation with interferon (IFN)-gamma. Cells expressed the proinflammatory mediator macrophage migration inhibitory factor (MIF). All NADPH oxidase subunits were constitutively present, but nitroblue tetrazolium reduction was only detectable upon activation with IFN-gamma. SigM5 monoblasts were sensitive to arsenic trioxide (As2O3) previously not described to induce apoptosis in monoblastic cells. Differing considerably in morphology, immunophenotype and sensitivity to arsenics from the widely used cell lines U937, HL-60 and THP-1, SigM5 is a new monoblastic cell line useful for studying leukaemogenesis, monocyte differentiation and tumour cell susceptibility to arsenic compounds.
迄今为止，已鉴定的人类单核细胞系寥寥无几。我们从一名急性单核细胞白血病（FAB M5a）患者中建立了SigM5细胞系。原始白血病细胞的核型为47,XY,+8，而该细胞系显示出一个主干克隆81,XX,Y,Y,1,4,6,7,+8,+8,9,10,10,11,13,16,19[cp]，同时也存在一个次要的旁系克隆。细胞化学染色显示，α-萘丁酸醋酸酯酶呈强阳性，苏丹黑呈颗粒状阳性，髓过氧化物酶呈弱阳性。细胞对CD13、CD15、CD18、CD23、CD33、CD38、CD45、CD68和髓过氧化物酶呈阳性反应。CD14表达率为3%-15%。在用干扰素（IFN）-γ刺激后，SigM5组成性分泌白细胞介素（IL）-2、IL-8、IL-10、肿瘤坏死因子（TNF）-α、铁蛋白、溶菌酶、N-弹性蛋白酶和新蝶呤。细胞表达促炎介质巨噬细胞迁移抑制因子（MIF）。所有NADPH氧化酶亚基均组成性存在，但仅在用IFN-γ激活后才能检测到硝基蓝四唑还原。SigM5单核细胞对三氧化二砷（As2O3）敏感，此前未发现三氧化二砷能诱导单核细胞凋亡。SigM5在形态、免疫表型和对砷的敏感性方面与广泛使用的细胞系U937、HL-60和THP-1有很大差异，是一种用于研究白血病发生、单核细胞分化和肿瘤细胞对砷化合物敏感性的新型单核细胞系。
