Oyajobi B O, Deng J H, Dallas S L, Jenson H B, Mundy G R, Gao S J
Departments of Medicine, Pediatrics and Microbiology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229-3900, USA.
Br J Haematol. 2000 May;109(2):413-9. doi: 10.1046/j.1365-2141.2000.02038.x.
Kaposi's sarcoma-associated herpesvirus (KSHV, also known as HHV-8) has been found in patients with multiple myeloma (MM) and postulated to be aetiologically associated with the development of this common plasma cell malignancy. A murine model of MM was previously established in which intravenous transfer of 5T myeloma cells into C57BL/KaLwRij mice resulted in characteristic features of human MM. In the present study, we sought to identify herpesvirus DNA sequences in this murine model of MM through polymerase chain reaction (PCR) analysis using primers specific for KSHV, murine herpesvirus 68 (MHV68) and murine cytomegalovirus (MCMV) as well as consensus primers designed from the highly conserved DNA polymerase genes of the Herpesviridae family. None of the DNA samples from whole bone marrow (n = 6) or dendritic cells enriched by long-term culture (n = 8) of 5T myeloma-bearing mice as well as the 5T myeloma cell lines (n = 3) maintained in long-term culture yielded specific amplification products in any of the PCR assays. Two KSHV-specific serological assays measuring antibodies to KSHV latent and lytic antigens also failed to detect the presence of anti-KSHV antibodies in mice that developed MM. These results suggest that the development of 5T murine MM is unlikely to be involved with KSHV or a KSHV-like murine herpesvirus.
卡波西肉瘤相关疱疹病毒（KSHV，也称为HHV - 8）已在多发性骨髓瘤（MM）患者中被发现，并被推测与这种常见浆细胞恶性肿瘤的发生在病因学上相关。先前已建立了一种MM小鼠模型，其中将5T骨髓瘤细胞静脉注射到C57BL/KaLwRij小鼠体内会导致出现人类MM的特征。在本研究中，我们试图通过聚合酶链反应（PCR）分析，使用针对KSHV、鼠疱疹病毒68（MHV68）和鼠巨细胞病毒（MCMV）的特异性引物以及根据疱疹病毒科高度保守的DNA聚合酶基因设计的通用引物，来鉴定该MM小鼠模型中的疱疹病毒DNA序列。来自荷5T骨髓瘤小鼠的全骨髓（n = 6）或经长期培养富集的树突状细胞（n = 8）以及长期培养的5T骨髓瘤细胞系（n = 3）的DNA样本，在任何PCR检测中均未产生特异性扩增产物。两种检测针对KSHV潜伏和裂解抗原抗体的KSHV特异性血清学检测方法，也未能在发生MM的小鼠中检测到抗KSHV抗体的存在。这些结果表明，5T小鼠MM的发生不太可能与KSHV或类似KSHV的鼠疱疹病毒有关。
