Holden H T, Haskill J S, Kirchner H, Herberman R B
J Immunol. 1976 Aug;117(2):440-6.
The ability of cells from primary MSV-induced tumors to function as effector cells in vitro was evaluated. Host cells were isolated by enzymatic disaggregation of the tumor and fractionated by sedimentation velocity at unit gravity on a Ficoll gradient. Characterization of these cells indicated that 30 to 40 % were T lymphocytes, about 50% were macrophages and less than 5% were B lymphocytes. Two different functional activities were mediated by these cells: cytolysis, as measured by the CRA, and inhibition of proliferation, as measured by the GIA. The effector cells in the CRA were T cells with sedimentation velocities of 3.5 to 4.0 mm/hr, whereas those cells which mediated the GIA were presumably macrophages and displayed a heterogeneity in size two peak sedimentation velocities, one at 4.0 mm/hr and another at 6.0 mm/hr. Activity by the effector cells in the CRA was antigen specific in contrast to the activity in the GIA which was directed against cells which did not carry detectable cross-reacting antigens.
对原发性MSV诱导肿瘤的细胞在体外作为效应细胞发挥功能的能力进行了评估。通过酶解肿瘤分离宿主细胞，并在Ficoll梯度上以单位重力沉降速度进行分级分离。对这些细胞的表征表明，30%至40%为T淋巴细胞，约50%为巨噬细胞，不到5%为B淋巴细胞。这些细胞介导两种不同的功能活性：通过细胞毒活性（CRA）测定的细胞溶解作用，以及通过生长抑制活性（GIA）测定的增殖抑制作用。CRA中的效应细胞是沉降速度为3.5至4.0毫米/小时的T细胞，而介导GIA的细胞可能是巨噬细胞，并且在大小上表现出异质性，有两个峰值沉降速度，一个在4.0毫米/小时，另一个在6.0毫米/小时。与GIA中针对不携带可检测交叉反应抗原的细胞的活性相反，CRA中效应细胞的活性是抗原特异性的。
