Thirkettle I, Harvey P, Hasleton P S, Ball R Y, Warn R M
Department of Histopathology/Cytopathology, Norfolk & Norwich Hospital, School of Biological Sciences, University of East Anglia, UK.
Histopathology. 2000 Jun;36(6):522-8. doi: 10.1046/j.1365-2559.2000.00888.x.
To assess the immunoreactivity of malignant mesotheliomas for N- and E-cadherins, hepatocyte growth factor/scatter factor (HGF/SF) and the tyrosine kinase receptors, met and erbB-2.
Pleural malignant mesotheliomas were stained using a standard indirect immunoperoxidase method applied to paraffin sections. Malignant mesotheliomas were immunoreactive for N-cadherin (26/29; 90%), met (29/29; 100%) and erbB-2 (28/29; 97%). Focal immunoreactivity was present for E-cadherin in epithelioid or mixed tumours (14/25; 56%), and for HGF/SF (9/24; 38%).
Expression of N-cadherin supports the diagnosis of malignant mesothelioma and use of appropriate antibodies would be a useful addition to a diagnostic antibody panel. Focal staining for E-cadherin does not exclude mesothelioma. Signalling pathways mediated via met and erbB-2 may play a role in the growth and spread of malignant mesotheliomas.
评估恶性间皮瘤对N-钙黏蛋白、E-钙黏蛋白、肝细胞生长因子/扩散因子（HGF/SF）以及酪氨酸激酶受体met和erbB-2的免疫反应性。
采用标准间接免疫过氧化物酶法对胸膜恶性间皮瘤石蜡切片进行染色。恶性间皮瘤对N-钙黏蛋白（26/29；90%）、met（29/29；100%）和erbB-2（28/29；97%）呈免疫反应性。在上皮样或混合型肿瘤中，E-钙黏蛋白呈局灶性免疫反应性（14/25；56%），HGF/SF呈局灶性免疫反应性（9/24；38%）。
N-钙黏蛋白的表达支持恶性间皮瘤的诊断，使用合适的抗体将有助于补充诊断抗体组。E-钙黏蛋白的局灶性染色不能排除间皮瘤。通过met和erbB-2介导的信号通路可能在恶性间皮瘤的生长和扩散中起作用。
