Yukawa Y, Sugita M, Choisne N, Small I, Sugiura M
Center for Gene Research, Nagoya University, Nagoya 464-8602, Japan.
Plant J. 2000 Jun;22(5):439-47. doi: 10.1046/j.1365-313x.2000.00752.x.
The effect of alteration of 5' and 3' flanking sequences on the transcription of plant tRNA genes was analysed using an RNA polymerase III-dependent in vitro transcription system derived from nuclei of cultured tobacco cells. A TATA-like sequence and the CAA motif frequently observed upstream of plant tRNA genes, and the poly(T) stretch usually present downstream, were shown to be necessary for efficient re-initiation of transcription. The CAA motif was shown to be a transcription initiation site. Introduction of the CAA and TATA-like motifs into a gene naturally lacking them greatly enhanced transcription by promoting efficient re-initiation.
利用源自培养烟草细胞核的RNA聚合酶III依赖的体外转录系统，分析了5'和3'侧翼序列改变对植物tRNA基因转录的影响。植物tRNA基因上游常见的类TATA序列和CAA基序，以及通常位于下游的聚（T）序列，被证明是转录有效重新起始所必需的。CAA基序被证明是一个转录起始位点。将CAA和类TATA基序引入天然缺乏它们的基因中，通过促进有效重新起始极大地增强了转录。
