Gourley I S, Wu J, Bassinger S, Montoya G D, Yee J, Griffith B B, Kearns J, McKeen M, Birkos S, Williams T M, Kamoun M
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19305, USA.
Tissue Antigens. 2000 Apr;55(4):374-7. doi: 10.1034/j.1399-0039.2000.550413.x.
Sequence-specific oligonucleotide probe hybridization and sequence-specific primer polymerase chain reaction (PCR) typing suggested the presence of variants of HLA-B40 in three individuals. Two were part of 3,500 potential marrow donors being screened for the National Marrow Donor Program, while the third was a clinical specimen. PCR products encompassing HLA-B locus exons 1 through 3 were prepared and subcloned. In one individual, a native of the Pacific Islands, sequencing revealed a novel HLA-B40 allele (B4023). In two other individuals, a previously unknown exon 1 sequence was determined for HLA-B4016 (ethnicity unknown) and B*4020 (Hispanic). These findings further illustrate the substantial genetic variation present at the HLA-B locus within human populations.
