Eminović-Behrem S, Trobonjaca Z, Petrovecki M, Dobi-Babić R, Dujmović M, Jonjić N
Department of Pathology, Rijeka University School of Medicine, Croatia,
Croat Med J. 2000 Jun;41(2):154-8.
To investigate the prognostic significance of DNA ploidy and silver stained nucleolar organizer regions (AgNOR), as well as their relation to the histological grade and Dukes' stages of colorectal carcinomas, and the relation of tumor cells proportion in the S-phase and Dukes' stage, histologic grade, DNA ploidy, or AgNOR count.
DNA flow cytometric analysis and AgNOR were performed on 94 surgically removed colorectal carcinomas. The mean AgNOR count was calculated in 200 tumor cells for each case. Survival rates and tests for significance were evaluated using the log-rank test and Cox regression model.
There were no significant correlations between the ploidy pattern, histological grade, and Dukes' stage. Diploid tumors had a significantly lower AgNOR count (median 2.5, range 2.1-7.7) than aneuploid (median 6.2, range 2.0-7.9). Dukes' C stage tumors exhibited higher AgNOR count than Dukes' A or B stages. The proportion of tumor cells in S-phase did not correlate with any other parameter. Each of these parameters failed to show any correlation with survival. After dividing the tumors into those with high (>5) and low AgNOR count (<5), no correlation was found in the latter group between AgNOR and any other studied parameters, whereas in the group with high AgNOR count correlations to Dukes' stage, DNA ploidy, and histological grade were established.Conclusions. The difference in survival between well, moderately, and poorly differentiated tumors were significant in the group with high AgNOR counts. Dukes' C stage and aneuploid tumors had the worst prognosis.
探讨DNA倍体和银染核仁组成区（AgNOR）的预后意义，以及它们与结直肠癌组织学分级和Dukes分期的关系，还有肿瘤细胞S期比例与Dukes分期、组织学分级、DNA倍体或AgNOR计数的关系。
对94例手术切除的结直肠癌进行DNA流式细胞术分析和AgNOR检测。计算每例200个肿瘤细胞的平均AgNOR计数。采用对数秩检验和Cox回归模型评估生存率及显著性检验。
倍体模式、组织学分级和Dukes分期之间无显著相关性。二倍体肿瘤的AgNOR计数（中位数2.5，范围2.1 - 7.7）显著低于非整倍体肿瘤（中位数6.2，范围2.0 - 7.9）。Dukes C期肿瘤的AgNOR计数高于Dukes A或B期。肿瘤细胞S期比例与其他任何参数均无相关性。这些参数均未显示与生存率有任何相关性。将肿瘤分为AgNOR计数高（>5）和低（<5）两组后，低AgNOR计数组中AgNOR与任何其他研究参数均无相关性，而高AgNOR计数组中AgNOR与Dukes分期、DNA倍体和组织学分级存在相关性。结论：AgNOR计数高的组中，高分化、中分化和低分化肿瘤的生存率差异显著。Dukes C期和非整倍体肿瘤预后最差。
