Inoue H, Mimori K, Shiraishi T, Kataoka A, Sadanaga N, Ueo H, Barnard G F, Mori M
Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan.
Oncol Rep. 2000 Jul-Aug;7(4):871-4. doi: 10.3892/or.7.4.871.
Tissue inhibitor of metalloproteinase-1 (TIMP-1) is an inhibitor of matrix metalloproteinase (MMP) in human carcinomas. TIMP-1 is thus considered to inhibit carcinoma invasion and metastasis. On the other hand, recent reports have disclosed that TIMP-1 also possesses a growth-promoting function. The clinical significance of TIMP-1 expression has not been fully determined in breast carcinoma. We thus examined the expression of TIMP-1 mRNA in tumor tissues of 100 breast carcinoma cases by a reverse transcriptase-polymerase chain reaction assay. The expression of TIMP-1 mRNA in each case was evaluated semi-quantitatively with adjustment for the TIMP-1 expression in a control breast carcinoma cell line, MCF7. There was a significant inverse correlation between the TIMP-1 expression and lymph node metastasis (p<0.05). A multivariate analysis disclosed that TIMP-1 expression status was an independent determinant factor for lymph node metastasis. In addition, the tumors with positive estrogen or progesterone receptors showed a higher TIMP-1 mRNA expression than those without the receptors, but this did not reach statistical significance. The findings suggest that the breast tumors with high TIMP-1 expression might show less malignant potential than those with low TIMP-1 expression.
金属蛋白酶组织抑制剂-1（TIMP-1）是人类癌症中基质金属蛋白酶（MMP）的一种抑制剂。因此，TIMP-1被认为可抑制癌症的侵袭和转移。另一方面，最近的报道显示TIMP-1还具有促进生长的功能。TIMP-1表达在乳腺癌中的临床意义尚未完全明确。因此，我们通过逆转录聚合酶链反应分析检测了100例乳腺癌病例肿瘤组织中TIMP-1 mRNA的表达。根据对照乳腺癌细胞系MCF7中的TIMP-1表达情况，对每个病例中TIMP-1 mRNA的表达进行半定量评估。TIMP-1表达与淋巴结转移之间存在显著的负相关（p<0.05）。多因素分析显示，TIMP-1表达状态是淋巴结转移的一个独立决定因素。此外，雌激素或孕激素受体阳性的肿瘤比无受体的肿瘤显示出更高的TIMP-1 mRNA表达，但这未达到统计学意义。这些发现表明，TIMP-1高表达的乳腺肿瘤可能比TIMP-1低表达的肿瘤具有更低的恶性潜能。
