Department of Breast Surgery, Affiliated Third Hospital of Harbin Medical University, 6 Baojian Road, Harbin, China.
Breast. 2013 Jun;22(3):330-4. doi: 10.1016/j.breast.2012.08.002. Epub 2012 Sep 18.
We examined mRNA expression for MMP-2, MMP-7, MMP-9, MT1-MMP, TIMP-1, and TIMP-2 in human breast cancer tissues, and the association between their expression and clinicopathological variables.
Breast tissue samples from 120 patients with breast cancer were available for this study. To determine mRNA expression for MMP-2, MMP-7, MMP-9, MT1-MMP, TIMP-1, and TIMP-2, semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was carried out on tumor and normal tissues, respectively.
Mean MMP-2, MMP-7, MMP-9, MT1-MMP, TIMP-1, and TIMP-2 mRNA expression in the breast cancer was significantly higher than in the normal tissue. In terms of tumor size and lymph node metastasis of breast cancer, the differences in MMP-2, MMP-7, MMP-9, and MT1-MMP mRNA expression levels were significant.
The association between the increased expression of MMP-2, MMP-7, MMP-9, and MTI-MMP and clinicopathological parameters reflects a role in predicting the aggressive behavior of breast cancer.
我们检测了人乳腺癌组织中 MMP-2、MMP-7、MMP-9、MT1-MMP、TIMP-1 和 TIMP-2 的 mRNA 表达，并分析了它们的表达与临床病理变量之间的关系。
本研究共纳入 120 例乳腺癌患者的乳腺组织标本。采用半定量逆转录-聚合酶链反应（RT-PCR）方法分别检测肿瘤组织和正常组织中 MMP-2、MMP-7、MMP-9、MT1-MMP、TIMP-1 和 TIMP-2 的 mRNA 表达。
乳腺癌组织中 MMP-2、MMP-7、MMP-9、MT1-MMP、TIMP-1 和 TIMP-2 的 mRNA 表达水平明显高于正常组织。在乳腺癌的肿瘤大小和淋巴结转移方面，MMP-2、MMP-7、MMP-9 和 MT1-MMP mRNA 表达水平的差异具有统计学意义。
MMP-2、MMP-7、MMP-9 和 MT1-MMP 的表达增加与临床病理参数之间的关联反映了其在预测乳腺癌侵袭性行为方面的作用。
