Amici R, Domeniconi R, Jones C A, Morales-Cobas G, Perez E, Tavernese L, Torterolo P, Zamboni G, Parmeggiani P L
Dipartimento di Fisiologia Umana e Generale, Università di Bologna, Piazza di Porta S. Donato, 2, I-40127, Bologna, Italy.
Brain Res. 2000 Jun 23;868(2):241-50. doi: 10.1016/s0006-8993(00)02337-4.
The effects of the rhythmical delivery of an auditory stimulus (1000 Hz, from 50 to 100 dB, 20 ms, every 20 s) on the pattern of rapid eye movement (REM) sleep occurrence was studied in the rat. The stimulation was simultaneously carried out on pairs of rats over 5 consecutive days (10-h recording sessions), during which a tone of increasing intensity (50, 63, 75, 88, 100 dB) was used. In each experimental session, auditory stimulation was triggered by the REM sleep occurrence of one rat (REMS-selective stimulation) whilst the other rat simultaneously received the same stimuli, but during any stage of the wake-sleep cycle (REMS-unselective stimulation). The results showed that the total amount of REM sleep in the 10-h recording session was increased over the 5 days of stimulation in the REMS-unselective group. This effect was due to an increase in the mean duration of REM sleep episodes. However, no significant changes were observed in animals under REMS-selective stimulation, nor in a third group of animals in which the spontaneous evolution of REM sleep occurrence (REMS-spontaneous) was studied. Since 86% of the stimuli under the REMS-unselective auditory stimulation fell outside REM sleep, the result would suggest that REM sleep occurrence is affected when the stimuli are delivered during a time period (i.e. during wakefulness or non-REM sleep) in which it is well known that physiological regulations are fully operant.
研究了节律性给予听觉刺激（1000赫兹，强度从50分贝到100分贝，时长20毫秒，每20秒一次）对大鼠快速眼动（REM）睡眠发生模式的影响。在连续5天（每次记录时长10小时）内对成对的大鼠同时进行刺激，期间使用强度递增的音调（50、63、75、88、100分贝）。在每个实验时段，由一只大鼠的REM睡眠发生触发听觉刺激（REM睡眠选择性刺激），而另一只大鼠同时接受相同刺激，但在清醒 - 睡眠周期的任何阶段（REM睡眠非选择性刺激）。结果显示，在REM睡眠非选择性刺激组中，10小时记录时段内REM睡眠的总量在刺激的5天中有所增加。这种效应是由于REM睡眠时段的平均时长增加所致。然而，在REM睡眠选择性刺激下的动物以及研究REM睡眠发生自发演变（REM睡眠自发组）的第三组动物中，未观察到显著变化。由于在REM睡眠非选择性听觉刺激下86%的刺激发生在REM睡眠之外，结果表明当刺激在已知生理调节充分发挥作用的时间段（即清醒或非REM睡眠期间）给予时，REM睡眠的发生会受到影响。
