Yoon J H, Kim K S, Kim S S, Lee J G, Park I Y
Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea.
Ann Otol Rhinol Laryngol. 2000 Jun;109(6):594-601. doi: 10.1177/000348940010900612.
The purpose of this study was to subculture normal human nasal epithelial (NHNE) cells without compromising their ability to differentiate into secretory and ciliated cells and to study the effect of retinoic acid on mucous and serous secretions in passaged cells and to compare the expression of mucin and lysozyme in cultured cells with those in in vivo nasal epithelium. The subcultured cells were tested after every passage for secretory differentiation in air-liquid interface cultures. The cultured NHNE cells secreted mucin and lysozyme. The cells became squamous and mucin secretion decreased when retinoic acid was deleted from the culture media. Cells from passage 1 through passage 2 remained able to differentiate into mucous or squamous cells. Mucin gene 4 (MUC4), MUC5AC, MUC7, MUC8, and lysozyme messenger RNAs were expressed in passage 2 NHNE cells. In conclusion, passage 2 NHNE cell cultures retain features of normal epithelium and are suitable for many studies of upper airway cell biology.
本研究的目的是传代培养正常人鼻上皮（NHNE）细胞，同时不损害其分化为分泌细胞和纤毛细胞的能力；研究视黄酸对传代细胞中黏液和浆液分泌的影响；并比较培养细胞中黏蛋白和溶菌酶的表达与体内鼻上皮中的表达。每次传代后，在气液界面培养中测试传代培养的细胞的分泌分化情况。培养的NHNE细胞分泌黏蛋白和溶菌酶。当从培养基中去除视黄酸时，细胞变为鳞状，黏液分泌减少。第1代至第2代的细胞仍能够分化为黏液细胞或鳞状细胞。第2代NHNE细胞中表达了黏蛋白基因4（MUC4）、MUC5AC、MUC7、MUC8和溶菌酶信使核糖核酸。总之，第2代NHNE细胞培养物保留了正常上皮的特征，适用于许多上呼吸道细胞生物学研究。
