Lambrechts N, Picard J, Tustin R C
Department of Surgery, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
J S Afr Vet Assoc. 1999 Mar;70(1):40-2. doi: 10.4102/jsava.v70i1.749.
A systemic disease associated with pyrexia, lymphadenopathy, and arthropathy of several joints of the appendicular skeleton in a dog is described. Chlamydia-like organisms were detected on light-microscopic examination of a smear made from joint fluid aspirated from one of the affected joints. A group-specific lipopolysaccharide antigen shared by all Chlamydia spp. was demonstrated by direct fluorescent antibody staining of joint fluid, which also proved positive for chlamydia by means of the relevant polymerase chain reaction test. An indirect fluorescent antibody test on serum was also positive, although the complement fixation test was negative. Attempts to grow the organism from joint aspirates in the yolk sac of embryonating hens' eggs and on appropriate tissue cultures, however, failed. Chlamydia spp. are considered to have played an aetiological role in this case, making it the first substantiated case of naturally-occurring arthropathy in a dog due to chlamydiosis. The origin of the infection could not be traced.
本文描述了一只犬类出现的一种全身性疾病，该疾病伴有发热、淋巴结病以及四肢骨骼多个关节的关节病。在对从一个患病关节抽取的关节液涂片进行光学显微镜检查时，发现了衣原体样生物体。通过对关节液进行直接荧光抗体染色，证实了所有衣原体属共有的一种群特异性脂多糖抗原，并且通过相关聚合酶链反应试验也证明关节液衣原体呈阳性。血清间接荧光抗体试验也呈阳性，不过补体结合试验呈阴性。然而，尝试从关节抽取物在鸡胚卵黄囊中以及在合适的组织培养物中培养该生物体均告失败。衣原体属被认为在该病例中起到了病因作用，使其成为首例经证实的犬类因衣原体病导致的自然发生的关节病病例。感染源无法追踪。
