Matsuzaki S, Fukaya T, Uehara S, Murakami T, Sasano H, Yajima A
Tohoku University School of Medicine, Sendai, Japan.
Fertil Steril. 2000 Jun;73(6):1219-25. doi: 10.1016/s0015-0282(00)00527-6.
To evaluate the expression of estrogen receptor (ER)-alpha and ER-beta messenger RNA (mRNA) in ovarian endometriosis.
Prospective study.
University hospital.
PATIENT(S): Patients with endometriosis and patients with uterine leiomyoma or carcinoma in situ.
INTERVENTION(S): A sample of ovarian endometriotic cyst tissue was obtained from each of the 32 patients during laparoscopic cystectomy. Samples of ovarian tissue and endometrium were obtained from 15 patients during or just after surgery.
MAIN OUTCOME MEASURE(S): Expression of mRNA for ER-alpha and ER-beta with use of the reverse transcription-polymerase chain reaction (RT-PCR) and nonradioactive in situ hybridization (ISH) techniques.
RESULT(S): Expression of mRNA for ER-alpha and ER-beta was observed in all of the control tissues from the normal endometrium and normal ovaries in the RT-PCR and ISH analyses, although the distribution of positive signals changed in the ISH analysis during different phases of the menstrual cycle. Messenger RNA for ER-alpha was detected in all of the ovarian endometriotic cysts analyzed (19 of 19), but mRNA for the ER-beta was limited (12 of 19) in the RT-PCR analysis. The ISH analysis confirmed the RT-PCR results and revealed that the two estrogen receptors were localized in both epithelial and stromal cells of endometriotic tissues.
CONCLUSION(S): The results suggest that predominant expression of ER-alpha in both glandular epithelial and stromal cells may be essential to the development and growth of ovarian endometriosis.
评估雌激素受体（ER）-α和ER-β信使核糖核酸（mRNA）在卵巢子宫内膜异位症中的表达。
前瞻性研究。
大学医院。
子宫内膜异位症患者以及子宫平滑肌瘤或原位癌患者。
在32例患者进行腹腔镜囊肿切除术时，从每位患者获取卵巢子宫内膜异位囊肿组织样本。在手术期间或刚结束后，从15例患者获取卵巢组织和子宫内膜样本。
运用逆转录-聚合酶链反应（RT-PCR）和非放射性原位杂交（ISH）技术检测ER-α和ER-β的mRNA表达。
在RT-PCR和ISH分析中，正常子宫内膜和正常卵巢的所有对照组织均观察到ER-α和ER-β的mRNA表达，尽管在月经周期的不同阶段，ISH分析中阳性信号的分布有所变化。在所有分析的卵巢子宫内膜异位囊肿（19个中的19个）中均检测到ER-α的mRNA，但在RT-PCR分析中，ER-β的mRNA表达受限（19个中的12个）。ISH分析证实了RT-PCR结果，并显示两种雌激素受体定位于异位内膜组织的上皮细胞和基质细胞中。
结果表明，ER-α在腺上皮细胞和基质细胞中的优势表达可能对卵巢子宫内膜异位症的发生和发展至关重要。
