Frolova G M, Kuznetsova T A, Mikhaĭlov V V, Eliakov G B
Pacific Institute of Bioorganic Chemistry, Far-Eastern Division, Russian Academy of Sciences, Vladivostok, Russia.
Bioorg Khim. 2000 Apr;26(4):315-20.
A competitive ELISA using the intact toxin as a coating antigen for detecting palytoxin was developed. This immunoassay allows palytoxin (PTX) to be determined in the range of 6-250 ng/ml. In sensitivity, this determination is comparable with RIA but is three times inferior to ELISA using monoclonal antibodies. Inhibition experiments using some toxins of marine invertebrates proved the serological specificity of the palytoxin binding to antibodies. Both the indirect and competitive ELISA were used to find PTX-producing bacteria among 420 isolates of sea bacteria. It was found that gram-negative bacteria Aeromonas sp. and Vibrio sp. associated with toxic samples of the soft coral Palythoa sp. produced compounds antigenically related to PTX.
开发了一种竞争性酶联免疫吸附测定法（ELISA），使用完整毒素作为包被抗原检测岩沙海葵毒素。这种免疫测定法可测定浓度范围为6 - 250 ng/ml的岩沙海葵毒素（PTX）。在灵敏度方面，该测定法与放射免疫分析（RIA）相当，但比使用单克隆抗体的ELISA低三倍。使用一些海洋无脊椎动物毒素进行的抑制实验证明了岩沙海葵毒素与抗体结合的血清学特异性。间接ELISA和竞争性ELISA均用于在420株海洋细菌分离物中寻找产生PTX的细菌。结果发现，与软珊瑚岩沙海葵属有毒样本相关的革兰氏阴性菌气单胞菌属和弧菌属产生了与PTX抗原相关的化合物。
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