斑节对虾毒素：一种海葵毒素的荧光结构异构体。

Lophozozymus pictor toxin: a fluorescent structural isomer of palytoxin.

作者信息

Lau C O, Tan C H, Khoo H E, Yuen R, Lewis R J, Corpuz G P, Bignami G S

机构信息

Department of Biochemistry, Faculty of Medicine, National University of Singapore.

出版信息

Toxicon. 1995 Oct;33(10):1373-7. doi: 10.1016/0041-0101(95)00075-w.

DOI:10.1016/0041-0101(95)00075-w

PMID:8599188

Abstract

Purified Lophozozymus pictor toxin (LPTX) shares many properties similar to palytoxin (PTX). LPTX and palytoxin isolated from Palythoa caribaeorum (C-PTX) have similar mol. wts of approx. 2680 on ionspray mass spectrometry (MS). In addition, antibodies against PTX could recognize and bind LPTX. Mixed mode high-performance liquid chromatography (HPLC) of LPTX, C-PTX and H-PTX (isolated from Palythoa tuberculosa) showed a major PTX component common to all three with the characteristic PTX-like UV spectrum at a retention time (Rt) of 17 min. However, LPTX exhibits fluorescence but PTX of equivalent toxicity does not. LPTX showed a unique peak at Rt of approx. 22 min on mixed mode HPLC. In addition, LPTX and C-PTX showed different ion fragmentation patterns on MS/MS. These results suggest that LPTX and the palytoxins are structural isomers, containing at least one difference which gives rise to fluorescence in LPTX.

摘要

纯化的斑纹海葵毒素（LPTX）具有许多与岩沙海葵毒素（PTX）相似的特性。从加勒比海葵（Palythoa caribaeorum）中分离出的LPTX和岩沙海葵毒素（C-PTX）在离子喷雾质谱（MS）上具有相似的分子量，约为2680。此外，抗PTX抗体能够识别并结合LPTX。对LPTX、C-PTX和从结节海葵（Palythoa tuberculosa）中分离出的H-PTX进行混合模式高效液相色谱（HPLC）分析，结果显示这三种毒素都有一个主要的PTX成分，其在保留时间（Rt）为17分钟时具有典型的类PTX紫外光谱。然而，LPTX具有荧光，而同等毒性的PTX则没有。在混合模式HPLC上，LPTX在Rt约为22分钟处显示出一个独特的峰。此外，LPTX和C-PTX在MS/MS上显示出不同的离子碎片模式。这些结果表明，LPTX和岩沙海葵毒素是结构异构体，至少存在一个差异，使得LPTX产生荧光。