Kato S, Yamaguchi H, Shimamoto Y, Mikami T
Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
Hereditas. 2000;132(1):43-8. doi: 10.1111/j.1601-5223.2000.00043.x.
A physical map of the azuki bean (Vigna angularis cv. Erimo-shozu) chloroplast DNA (cpDNA) was constructed by localising the cleavage sites of PstI, SalI, SmaI, SacI, KpnI, PvuII and XhoI. The resulting map is more similar to the cpDNA-maps of two Vigna species (mung bean, V. radiata, and V. nakashimae) than to common bean (Phaseolus vulgaris) cpDNA map. Azuki bean was originally classified in the genus Phaseolus, and the inclusion of this taxon in the genus Vigna is a recent taxonomic decision. Our result is thus in favour of the taxonomic placement of azuki bean in the same genus as V. nakashimae and mung bean. We also found that a weed-form accession of azuki bean has a 96-bp deletion relative to the cultivar Erimo-shozu. The 96-bp deletion is located between the trnS-UGA and psbC genes in the large single-copy region of the chloroplast genome. This deletion is flanked by imperfect 9-bp direct repeats, suggesting that the deletion was a result of intra-molecular recombination mediated by these direct repeats.
通过定位限制性内切酶PstI、SalI、SmaI、SacI、KpnI、PvuII和XhoI的切割位点，构建了小豆（Vigna angularis cv. Erimo-shozu）叶绿体DNA（cpDNA）的物理图谱。所得图谱与两种豇豆属物种（绿豆，V. radiata，和V. nakashimae）的cpDNA图谱比与菜豆（Phaseolus vulgaris）的cpDNA图谱更为相似。小豆最初被归类于菜豆属，将该分类单元归入豇豆属是最近的分类学决定。因此，我们的结果支持将小豆与V. nakashimae和绿豆置于同一属的分类学定位。我们还发现，一种杂草形态的小豆种质相对于品种Erimo-shozu有一个96 bp的缺失。该96 bp缺失位于叶绿体基因组大单拷贝区域的trnS-UGA和psbC基因之间。该缺失两侧为不完美的9 bp直接重复序列，表明该缺失是由这些直接重复序列介导的分子内重组的结果。
