Campbell V, Lynch M A
Department of Physiology, Trinity College, Dublin, Ireland.
Cytokine. 2000 May;12(5):487-90. doi: 10.1006/cyto.1999.0584.
We recently demonstrated that the pro-inflammatory cytokine, interleukin 1beta (IL-1beta) elevates intracellular free Ca2+ levels ([Ca2+]i) in rat cortical synaptosomes in a manner involving activation of the IL-1 receptor and stimulation of p42 mitogen-activated protein (MAP) kinase. We now report that the effects of IL-1beta on [Ca2+]i are mimicked by the sphingolipid metabolite ceramide. In cortical synaptosomes ceramide elevates [Ca2+]i in a p42 MAP kinase-dependent manner, and we conclude that the effects of IL-1beta on Ca2+ homeostasis involve ceramide as an upstream component of the p42 MAP kinase pathway.
我们最近证明，促炎细胞因子白细胞介素1β（IL-1β）通过激活IL-1受体和刺激p42丝裂原活化蛋白（MAP）激酶的方式，提高大鼠皮质突触体中的细胞内游离Ca2+水平（[Ca2+]i）。我们现在报告，鞘脂代谢产物神经酰胺可模拟IL-1β对[Ca2+]i的影响。在皮质突触体中，神经酰胺以p42 MAP激酶依赖性方式提高[Ca2+]i，并且我们得出结论，IL-1β对Ca2+稳态的影响涉及神经酰胺作为p42 MAP激酶途径的上游成分。
