紫外线、N-甲基-N'-硝基-N-亚硝基胍和吖啶黄对游离型和细胞内型噬藻体AS-1的诱变作用
Mutagenesis of free and intracellular cyanophage AS-1 by ultraviolet, N-methanyl-N'-nitro-N-nitrosoguanidine and acriflavine.
作者信息
Amla D V
出版信息
Mutat Res. 1979 Feb;59(2):147-55. doi: 10.1016/0027-5107(79)90152-0.
Mutagenic actions of ultraviolet irradiation (UV), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and acriflavine (photodynamic) were tested in free and intracellular phage AS-1 infecting Anacystis nidulans IU625. Spontaneous and induced mutations with particular reference to host range (h) and minute plaque formation (m) were investigated. The spontaneous mutation frequencies varied from 10(-9) to 10(-8) and from 2 X 10(-5) to 2 X 10(-4) for h and m mutants respectively. UV was efficient in inducing h and m markers in free phage particles as well as intracellular phage; MNNG induced both markers in intracellular phage only, and acriflavine induced m mutants only in free as well as in infecting phages. UV-induced mutations in free phage were photo-reactivable by visible light. With all the mutagens used, maximal induction of both markers was observed with treatment of 2-h complexes.
在感染聚球藻属蓝细菌IU625的游离和细胞内噬菌体AS-1中测试了紫外线照射（UV）、N-甲基-N'-硝基-N-亚硝基胍（MNNG）和吖啶黄（光动力）的诱变作用。研究了自发和诱导突变，特别关注宿主范围（h）和微小噬菌斑形成（m）。h和m突变体的自发突变频率分别为10^(-9)至10^(-8)和2×10^(-5)至2×10^(-4)。UV在游离噬菌体颗粒以及细胞内噬菌体中有效诱导h和m标记；MNNG仅在细胞内噬菌体中诱导这两种标记，而吖啶黄仅在游离噬菌体以及感染噬菌体中诱导m突变体。游离噬菌体中UV诱导的突变可被可见光光复活。使用所有诱变剂时，处理2小时的复合物时观察到两种标记的最大诱导。
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