Haan C, Hermanns H M, Heinrich P C, Behrmann I
Department of Biochemistry, RWTH Aachen, Pauwelsstr. 30, 52074 Aachen, Germany.
Biochem J. 2000 Jul 1;349(Pt 1):261-6. doi: 10.1042/0264-6021:3490261.
gp130 is the common signal-transducing receptor chain of interleukin (IL)-6-type cytokines. Here we describe, for the first time, a single amino acid substitution (Trp(666)-->Ala) in the membrane-proximal interbox1/2 region that abrogates activation of STAT (signal transducer and activator of transcription) transcription factors and the proliferative response of pro-B-cell transfectants. Moreover, association of the Janus kinase JAK1 is prevented. No signalling of heterodimeric IL-5 receptor (IL-5R)/gp130 chimaeras occurs in COS-7 cells, even when only a single cytoplasmic chain of a gp130 dimer contains the Trp(666)Ala mutation, indicating that it acts dominantly.
gp130是白细胞介素（IL）-6型细胞因子的共同信号转导受体链。在此我们首次描述了膜近端框间1/2区域的一个单氨基酸取代（色氨酸（Trp）（666）→丙氨酸（Ala）），该取代消除了信号转导子和转录激活子（STAT）转录因子的激活以及前B细胞转染子的增殖反应。此外，Janus激酶JAK1的结合也被阻止。即使gp130二聚体中只有一条胞质链含有Trp（666）Ala突变，异源二聚体白细胞介素-5受体（IL-5R）/gp130嵌合体在COS-7细胞中也不会发生信号传导，这表明该突变起主导作用。
