Fehér T, Bodrogi L
Endocrinol Exp. 1976;10(3):201-9.
A simple and accurate gas chromatographic method for the determination of testosterone in human urine is described. Radioactive testosterone was added to samples to correct methodological loss. A mild acid hydrolysis under protection of benzene layer gave a single estimate of testosterone glucuronate and sulphate. Paper chromatography and rechromatography on chromatoplate served for preliminary qurification. Testosterone was gas chromatographed as acetate and guantitated by the internal standard procedure and flame ionization detector. Results of control experiments, normal values for female and male subjects and estimations in patients with alterations of androgen hormone metabolism are presented.
本文描述了一种简单而准确的气相色谱法，用于测定人尿中的睾酮。向样品中加入放射性睾酮以校正方法学损失。在苯层保护下进行温和的酸水解，可对睾酮葡萄糖醛酸酯和硫酸盐进行单一评估。纸色谱法和在色谱板上的再色谱法用于初步纯化。睾酮以乙酸酯形式进行气相色谱分析，并通过内标法和火焰离子化检测器进行定量。给出了对照实验结果、女性和男性受试者的正常值以及雄激素激素代谢改变患者的测定结果。
