一种以Cr(III)为催化剂测定过氧化氢的流动注射化学发光方法。应用于微藻培养物中H₂O₂的测定。
A flow injection chemiluminescence method using Cr(III) as a catalyst for determining hydrogen peroxide. Application to H(2)O(2) determination in cultures of microalgae.
作者信息
Escobar R, García-Domínguez S, Guiraúm A, Montes O, Galván F, de La Rosa F F
机构信息
Department of Analytical Chemistry, University of Seville, Spain.
出版信息
Luminescence. 2000 May-Jun;15(3):131-5. doi: 10.1002/1522-7243(200005/06)15:3<131::AID-BIO554>3.0.CO;2-8.
Flow injection analysis has been applied to the determination of hydrogen peroxide produced by some different species of microalgae. The method is based on the luminol-H(2)O(2) chemiluminescence reaction using Cr(III) as a catalyst. Optimum experimental conditions for the method have been studied and trace amounts of hydrogen peroxide determined with detection limits of 4 10(-8) mol/L. The method using Cr(III) was compared with that using horseradish peroxidase as the catalyst.
流动注射分析已应用于测定某些不同种类微藻产生的过氧化氢。该方法基于以Cr(III)为催化剂的鲁米诺-H₂O₂化学发光反应。研究了该方法的最佳实验条件，并测定了痕量过氧化氢，检测限为4×10⁻⁸ mol/L。将使用Cr(III)的方法与使用辣根过氧化物酶作为催化剂的方法进行了比较。
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