Becker M J, Ray A, Andersson L C, MAKELA O
Eur J Immunol. 1975 Apr;5(4):262-6. doi: 10.1002/eji.1830050409.
A small bifunctional antigen (4-hydroxy-5-iodo-3-nitrophenyl)acetyl-epsilon-aminocaproyl-L-tyrosine-azobenzene-p-arsonate [NIP-cap-TYR(ABA)] was found to induce fair humoral antibody formation against NIP-cap but very little anti-ABA-TYR. This was observed in rats and guinea pigs. Prior immunization with ABA-TYR, either as such or coupled to dodecanoylated bovine serum albumin (lipid-BSA), primed rats for an enhanced anti-NIP response to NIP-cap-TYR(ABA). An attempt to encourage rats to produce anti-ABA-TYR in response to the bifunctional antigen by priming them with NIP-cap-lipid-BSA failed. Priming with ABA-TYR was dose-dependent. An injection of 1.5-15 nanomoles per rat primed for an increased production of anti-NIP while 150 nanomoles did not. Adult thymectomized x-irradiated rats had a poor anti-NIP response to the bifunctional antigen if they were reconstituted with T-enriched lymphoid cells from control mice, but a good response if reconstituted with similar cells from ABA-TYR-primed syngeneic rats.
一种小的双功能抗原（4-羟基-5-碘-3-硝基苯基）乙酰基-ε-氨基己酰-L-酪氨酸-偶氮苯-对砷酸盐[NIP-帽-TYR(ABA)]被发现能诱导针对NIP-帽的适度体液抗体形成，但针对抗ABA-TYR的抗体形成很少。这在大鼠和豚鼠中都有观察到。预先用ABA-TYR免疫，无论是单独使用还是与十二烷酰化牛血清白蛋白（脂质-BSA）偶联，都能使大鼠对NIP-帽-TYR(ABA)的抗NIP反应增强。试图通过用NIP-帽-脂质-BSA对大鼠进行预免疫来促使它们针对双功能抗原产生抗ABA-TYR的尝试失败了。用ABA-TYR进行预免疫是剂量依赖性的。每只大鼠注射1.5 - 15纳摩尔可引发抗NIP产量增加，而注射150纳摩尔则没有效果。成年胸腺切除并经X射线照射的大鼠，如果用来自对照小鼠的富含T细胞的淋巴细胞进行重建，对双功能抗原的抗NIP反应较差，但如果用来自经ABA-TYR预免疫的同基因大鼠的类似细胞进行重建，则反应良好。
