Yamagata E, Kamada A
Department of Biochemistry, Osaka Dental University, Japan.
J Osaka Dent Univ. 1999 Oct;33(2):83-7.
We investigated the mRNA expression of cell-surface heparan sulfate proteoglycans, syndecans and glypican, in the adult female monkey submandibular gland using the reverse transcription-polymerase chain reaction (RT-PCR) technique. Agarose gel electrophoresis of the PCR products of the cDNA generated from RNA was carried out to demonstrate the expression of mRNA in syndecan-1, syndecan-2, syndecan-4 and glypican in this study. In order to compare the mRNA expression level among the cell-surface heparan sulfate proteoglycans, we measured changes in the relative intensity of PCR products with increasing thermal cycle number. The expression levels were syndecan-4 > syndecan-1, syndecan-2 > glypican. Considering these results together with our previous report, we found that the cell-surface heparan sulfate proteoglycans, syndecan-1, syndecan-2, syndecan-4 and glypican, are synthesized in the monkey submandibular glands, and that their ectodomains are released into the extracellular matrix. It was speculated that control of the expression patterns of the cell-surface proteoglycans may regulate the cellular function and behavior in the submandibular gland.
我们使用逆转录-聚合酶链反应（RT-PCR）技术，研究了成年雌性猴下颌下腺中细胞表面硫酸乙酰肝素蛋白聚糖、多功能蛋白聚糖和磷脂酰肌醇蛋白聚糖的mRNA表达。对从RNA生成的cDNA的PCR产物进行琼脂糖凝胶电泳，以证明本研究中多功能蛋白聚糖-1、多功能蛋白聚糖-2、多功能蛋白聚糖-4和磷脂酰肌醇蛋白聚糖中mRNA的表达。为了比较细胞表面硫酸乙酰肝素蛋白聚糖之间的mRNA表达水平，我们测量了随着热循环次数增加PCR产物相对强度的变化。表达水平为多功能蛋白聚糖-4>多功能蛋白聚糖-1，多功能蛋白聚糖-2>磷脂酰肌醇蛋白聚糖。结合我们之前的报告综合考虑这些结果，我们发现细胞表面硫酸乙酰肝素蛋白聚糖，即多功能蛋白聚糖-1、多功能蛋白聚糖-2、多功能蛋白聚糖-4和磷脂酰肌醇蛋白聚糖，在猴下颌下腺中合成，并且它们的胞外域被释放到细胞外基质中。据推测，细胞表面蛋白聚糖表达模式的控制可能调节下颌下腺中的细胞功能和行为。
