人环孢素诱导的牙龈过度生长中细胞表面硫酸乙酰肝素蛋白聚糖的表达

Expression of cell-surface heparan sulfate proteoglycans in human cyclosporin-induced gingival overgrowth.

作者信息

Gnoatto N, Lotufo R F M, Matsuda M, Penna V, Marquezini M V

机构信息

Escola Bahiana de Medicina e Saúde Pública, Fundação Bahiana para o Desenvolvimento das Ciências, Salvador, Bahia, Brazil.

出版信息

J Periodontal Res. 2007 Dec;42(6):553-8. doi: 10.1111/j.1600-0765.2007.00981.x.

DOI:10.1111/j.1600-0765.2007.00981.x

PMID:17956469

Abstract

BACKGROUND AND OBJECTIVE

Cyclosporin A-induced gingival overgrowth comprises a variety of signaling pathways (including growth factors and proteoglycans) that are still not completely understood. In the present study, gingival overgrowth was investigated in transplant patients receiving cyclosporin A (cyclosporin A group) and compared with gingival tissues never exposed to the drug (control group) by analyzing the gene expression of the cell-surface heparan sulfate proteoglycans syndecan-2, syndecan-4 and betaglycan.

MATERIAL AND METHODS

mRNA analysis was carried out by reverse transcription-polymerase chain reaction amplification of pooled samples from nine patients of the cyclosporin A group and six control subjects. The groups were compared by the Student's t-test.

RESULTS

The expression of heparan sulfate proteoglycans was increased in the cyclosporin A group (165% for syndecan-2, 308% for syndecan-4, and 42% for betaglycan) compared with the control group.

CONCLUSION

Our findings agree with the current concept of cyclosporin A-induced gingival overgrowth and provide new evidence that its noncollagenous extracellular matrix is overexpressed.

摘要

背景与目的

环孢素A诱导的牙龈增生涉及多种信号通路（包括生长因子和蛋白聚糖），目前仍未完全明确。在本研究中，通过分析细胞表面硫酸乙酰肝素蛋白聚糖（syndecan - 2、syndecan - 4和betaglycan）的基因表达，对接受环孢素A治疗的移植患者（环孢素A组）的牙龈增生情况进行了研究，并与从未接触过该药物的牙龈组织（对照组）进行了比较。