Wolfensberger T J, Aptsiauri N, Godley B, Downes S, Bird A C
Hôpital Ophtalmique Jules Gonin, Université de Lausanne, Suisse.
Klin Monbl Augenheilkd. 2000 May;216(5):283-5. doi: 10.1055/s-2000-10561.
Recently, anti-Enolase and anti-carbonic anhydrase antibodies have been observed in over 60% of patients with retinitis pigmentosa (RP) and cystoid macular oedema (CME). We investigated the presence of these antibodies in a series of patients with CME due to different pathologies.
In 10 patients with CME serum antibodies against Carbonic anhydrase (CA) II (30 kD) and Enolase (46 kD) were sought using Western Blots, Dot Blots as well as ELISA.
Western and dot blotting showed anti-CA II antibodies in all and anti-Enolase antibodies in six of the 10 patients. The average titer measured with ELISA was 0.9 +/- 0.08 OD Units (0.35-1.4) with a dilution of 1:400.
The presence of anti-retinal antibodies in the serum of all patients confirms the high prevalence of these antibodies in patients with CME. This may suggest that a dysfunction of CA and enolase activity in the retinal pigment epithelium may lie at the root of oedema formation, whereas other mechanisms may be responsible in the absence of these antibodies.
最近，在超过60%的视网膜色素变性（RP）和黄斑囊样水肿（CME）患者中观察到抗烯醇化酶和抗碳酸酐酶抗体。我们调查了一系列因不同病理情况导致CME的患者中这些抗体的存在情况。
使用蛋白质印迹法、斑点印迹法以及酶联免疫吸附测定法（ELISA），在10例CME患者中寻找抗碳酸酐酶（CA）II（30 kD）和烯醇化酶（46 kD）的血清抗体。
蛋白质印迹法和斑点印迹法显示，所有10例患者中均存在抗CA II抗体，6例存在抗烯醇化酶抗体。ELISA测得的平均滴度为0.9 +/- 0.08 OD单位（0.35 - 1.4），稀释度为1:400。
所有患者血清中抗视网膜抗体的存在证实了这些抗体在CME患者中的高患病率。这可能表明视网膜色素上皮中CA和烯醇化酶活性的功能障碍可能是水肿形成的根源，而在没有这些抗体的情况下可能有其他机制起作用。
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