Ando K, Ogawa K, Li X, Kikugawa K
School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.
Biol Pharm Bull. 2000 Jun;23(6):689-94. doi: 10.1248/bpb.23.689.
4-Hydroxy-2(or 5)-ethyl-5(or 2)-methyl-3(2H)-furanone (HEMF) is representative of the Maillard reaction-derived reductones found in many foodstuffs. Influence of HEMF on iron ion-induced oxidative modification of human erythrocyte membranes and low density lipoprotein (LDL) under aerobic conditions was investigated. When human erythrocytes were incubated at 37 degrees C for 24 h with HEMF alone, levels of thiobarbituric acid-reactive substances (TBARS) and non-ionic detergent C12E8-insoluble membrane protein aggregates in the membranes were unchanged. Levels of TBARS and protein aggregates in the membranes increased on treatment of the cells with Fe(III) ion at 37 degrees C for 3 h were lowered in the presence of HEMF. Western blot analysis indicated that aggregates of band 3 protein induced by Fe (III) ion were decreased in the presence of HEMF, and the level of TBARS in LDL increased on treatment with Fe(II) or Fe(II) ion at 37 degrees C for 24 h was also lowered in the presence of HEMF. The results indicate that HEMF protected the iron ion-induced oxidative modification of human erythrocyte membranes and LDL.
4-羟基-2（或5）-乙基-5（或2）-甲基-3（2H）-呋喃酮（HEMF）是许多食品中发现的美拉德反应衍生的还原酮的代表。研究了HEMF在有氧条件下对铁离子诱导的人红细胞膜和低密度脂蛋白（LDL）氧化修饰的影响。当人红细胞在37℃下单独与HEMF孵育24小时时，膜中硫代巴比妥酸反应性物质（TBARS）和非离子洗涤剂C12E8不溶性膜蛋白聚集体的水平没有变化。在37℃下用Fe（III）离子处理细胞3小时后，膜中TBARS和蛋白聚集体的水平增加，而在HEMF存在下则降低。蛋白质印迹分析表明，在HEMF存在下，Fe（III）离子诱导的带3蛋白聚集体减少，并且在37℃下用Fe（II）或Fe（III）离子处理24小时后LDL中TBARS的水平在HEMF存在下也降低。结果表明，HEMF保护了铁离子诱导的人红细胞膜和LDL的氧化修饰。
