Maeda M, Kuwayama H, Yokoyama M, Nishio K, Morio T, Urushihara H, Katoh M, Tanaka Y, Saito T, Ochiai H, Takemoto K, Yasukawa H, Takeuchi I
Department of Biology, Graduate School of Science, Osaka University, Machikaneyama 1-16, Toyonaka, Osaka, 560-0043, Japan.
Dev Biol. 2000 Jul 1;223(1):114-9. doi: 10.1006/dbio.2000.9736.
We analyzed the spatial expression patterns of the genes involved in myosin function by in situ hybridization at the tipped aggregate and early culmination stages of Dictyostelium. Myosin heavy chain II mRNA was enriched in the anterior prestalk region of the tipped aggregates, whereas it disappeared from there and began to appear in both upper and lower cups of the early culminants. Similarly, mRNAs for essential light chain, regulatory light chain, myosin light chain kinase A, and myosin heavy chain kinase C were enriched in the prestalk region of the tipped aggregates. However, expression of these genes was distinctively regulated in the early culminants. These findings suggest the existence of mechanisms responsible for the expression of particular genes.
我们通过原位杂交技术，分析了盘基网柄菌在尖端聚集体和早期发育成熟阶段中，参与肌球蛋白功能的基因的空间表达模式。肌球蛋白重链II mRNA在尖端聚集体的前柄细胞区域富集，而在该区域消失，并开始出现在早期发育成熟体的上杯和下杯中。同样，必需轻链、调节轻链、肌球蛋白轻链激酶A和肌球蛋白重链激酶C的mRNA在尖端聚集体的柄细胞区域富集。然而，这些基因在早期发育成熟体中的表达受到独特的调控。这些发现表明存在负责特定基因表达的机制。
