Singer R S, Jeffrey J S, Carpenter T E, Cooke C L, Atwill E R, Johnson W O, Hirsh D C
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Vet Microbiol. 2000 Jul 3;75(1):59-71. doi: 10.1016/s0378-1135(00)00205-4.
Avian cellulitis in broiler chickens is primarily caused by Escherichia coli. Previous research found that the E. coli isolates of cellulitis origin were unique to each ranch, suggesting that these E. coli were endemic within the ranch environment. To test the hypothesis that the E. coli associated with cellulitis are endemic in the litter of the broiler house, we designed a study to determine whether E. coli DNA fingerprints associated with cellulitis persist over successive flocks that are grown in the same house. In addition, we assessed the impact of different cleaning and disinfection strategies on this persistence. Two broiler houses were followed on each of five farms over 3-4 flocks. A total of 353 E. coli isolates from cellulitis lesions were analyzed in this study, and 314 of these isolates (89%) were DNA fingerprinted by PFGE. In each ranch, there were several DNA fingerprint patterns that were present over successive flocks, regardless of the cleaning and disinfection strategy utilized. Isolates persisted as long as 191 days, implying that these E. coli are capable of persisting in the broiler house environment for long periods of time. In addition, these E. coli isolates were associated with cellulitis lesions in successive flocks. Thus, the isolates of E. coli that are associated with cellulitis in broiler chickens appear to be endemic in the litter environment of the broiler house.
肉鸡的禽蜂窝织炎主要由大肠杆菌引起。先前的研究发现，蜂窝织炎来源的大肠杆菌分离株在每个养殖场都是独特的，这表明这些大肠杆菌在养殖场环境中是地方性的。为了检验与蜂窝织炎相关的大肠杆菌在肉鸡舍垫料中是地方性的这一假设，我们设计了一项研究，以确定与蜂窝织炎相关的大肠杆菌DNA指纹在同一鸡舍中连续饲养的鸡群中是否持续存在。此外，我们评估了不同清洁和消毒策略对这种持续性的影响。在五个农场中的每个农场跟踪两个肉鸡舍，观察3至4批鸡群。本研究共分析了353株来自蜂窝织炎病变的大肠杆菌分离株，其中314株（89%）通过脉冲场凝胶电泳进行了DNA指纹分析。在每个养殖场，无论采用何种清洁和消毒策略，都有几种DNA指纹模式在连续的鸡群中出现。分离株持续存在长达191天，这意味着这些大肠杆菌能够在肉鸡舍环境中长期存在。此外，这些大肠杆菌分离株与连续鸡群中的蜂窝织炎病变有关。因此，与肉鸡蜂窝织炎相关的大肠杆菌分离株似乎在肉鸡舍的垫料环境中是地方性的。
