Lin L C, Kuo Y C, Chou C J
National Research Institute of Chinese Medicine, Taipei, Taiwan, Republic of China.
Planta Med. 2000 May;66(4):333-6. doi: 10.1055/s-2000-8540.
From the root of Limonium sinense (Girard) Ktze a new (2R,3S)-3,5,7,4'-tetrahydroxy-3',5'-dimethoxyflavanone was isolated and named isodihydrosyringetin (3), together with nine other known compounds, (-)-epigallocatechin 3-O-gallate (1), samarangenin B (2), myricetin (4), myricetin 3-O-alpha-rhamnopyranoside (5), quercetin 3-O-alpha-rhamnopyranoside (6), (-)-epigallocatechin (7), gallic acid (8), N-trans-caffeoyltyramine (9), and N-trans-feruloyltyramine (10). All of them were examined for their inhibitory effects on herpes simplex virus type-1 (HSV-1) replication in Vero cells. Both compounds 1 and 2 exhibited potent inhibitory activities in HSV-1 replication. Comparison of the IC50 values indicated that compounds 1 and 2 had higher inhibitory activities than the positive control acyclovir (38.6 +/- 2.6 vs. 55.4 +/- 5.3 microM, P < 0.001; 11.4 +/- 0.9 vs. 55.4 +/- 5.3 microM, P < 0.0005). Cytotoxicity was unlikely involved because no cell deaths were observable in the Vero cells following 5 day treatments with compound 1 or 2.
从中华补血草的根部分离得到一种新的(2R,3S)-3,5,7,4'-四羟基-3',5'-二甲氧基黄烷酮，命名为异二氢杨梅黄素(3)，同时还得到其他九种已知化合物，(-)-表没食子儿茶素3-O-没食子酸酯(1)、山柰酚B(2)、杨梅素(4)、杨梅素3-O-α-鼠李糖苷(5)、槲皮素3-O-α-鼠李糖苷(6)、(-)-表没食子儿茶素(7)、没食子酸(8)、N-反式-咖啡酰酪胺(9)和N-反式-阿魏酰酪胺(10)。检测了它们对非洲绿猴肾细胞中单纯疱疹病毒1型(HSV-1)复制的抑制作用。化合物1和2对HSV-1复制均表现出强效抑制活性。IC50值比较表明，化合物1和2的抑制活性高于阳性对照阿昔洛韦(38.6±2.6对55.4±5.3 μM，P<0.001；11.4±0.9对55.4±5.3 μM，P<0.0005)。不太可能涉及细胞毒性，因为用化合物1或2处理5天后，在非洲绿猴肾细胞中未观察到细胞死亡。
