Dumont F, Barrois R
Folia Biol (Praha). 1976;12(3):145-50.
Spleen cells from C3H/Hej mice (H-2k) respond poorly to the B-cell mitogen lipopolysaccharide in vitro as compared to the related strains C3H/Tif (H-2k) and CBA/Orl (H-2k). The electrokinetic properties of splenic lymphocytes from these 3 strains were investigated in parallel, in order to both quantitate low-mobility B cell and high-mobility T cell populations and measure their mean electrophoretic mobilities. C3H/Hej mice were found to possess the same proportion (55%) of LM cells as C3H/Tif and CBA/Orl mice. Therefore, the low LPS-responsiveness of C3H/Hej is not due to a numerical deficiency in B cells. Whereas the mean EPM of HM cells was identical in the 3 strains, that of LM cells was slightly (6%) but significantly (Student's t test, P less than 0.01) lower in C3H/Hej than in the high LPS-responder controls. This suggests that the membrane-structure required for activating interaction with LPS might contribute to B-cell electronegative surface-charge.
与相关品系C3H/Tif（H-2k）和CBA/Orl（H-2k）相比，C3H/Hej小鼠（H-2k）的脾细胞在体外对B细胞有丝分裂原脂多糖的反应较差。同时研究了这3个品系脾淋巴细胞的电动特性，以便对低迁移率B细胞群和高迁移率T细胞群进行定量，并测量它们的平均电泳迁移率。发现C3H/Hej小鼠拥有与C3H/Tif和CBA/Orl小鼠相同比例（55%）的低迁移率细胞。因此，C3H/Hej小鼠对脂多糖反应性低并非由于B细胞数量不足。虽然3个品系中高迁移率细胞的平均电泳迁移率相同，但C3H/Hej小鼠中低迁移率细胞的平均电泳迁移率比高脂多糖反应性对照小鼠略低（6%），但差异显著（学生t检验，P<0.01）。这表明与脂多糖激活相互作用所需的膜结构可能有助于B细胞的负表面电荷。
