Lallas T A, McClain S K, Shahin M S, Buller R E
Department of Obstetrics and Gynecology, The University of Iowa Hospitals and Clinics, Iowa City 52242, USA.
Cancer Epidemiol Biomarkers Prev. 2000 Jun;9(6):587-90.
Glutathione S-transferase mu-1 (GSTM1) is a polymorphic member of the mu class gene family of the glutathione S-transferases. Individuals who are GSTM1 null have increased susceptibility to lung and colon cancer. We hypothesized that: (a) GSTM1 null individuals might also be at increased risk for development of ovarian cancer; and (b) the GSTM1 genotype would influence response to chemotherapy. One hundred and forty-six individuals with invasive epithelial ovarian cancer were genotyped using a three-primer PCR reaction specific for the GSTM1 gene and an internal control glutathione S-transferase mu-4 (GSTM4). The products were analyzed on agarose gels. Healthy individuals without a family history of ovarian, breast, or colon cancer served as unmatched controls (n = 80). The results show that age at diagnosis, histological type, and stage of ovarian cancer were all independent of GSTM1 genotype. The frequency of the GSTM1 null genotype in the ovarian cancer cohort was similar to that in the control population, 51% versus 58%, P > 0.05. Likewise, median survival for individuals with advanced stage ovarian cancer was independent of GSTM1 genotype. We concluded that the GSTM1 null genotype does not increase ovarian cancer risk. These findings suggest that GSTM1 does not play a significant role in detoxifying environmental factors that influence ovarian carcinogenesis and does not play an important role in the resistance of ovarian cancer to chemotherapy.
谷胱甘肽S-转移酶μ-1（GSTM1）是谷胱甘肽S-转移酶μ类基因家族的一个多态性成员。GSTM1基因缺失的个体患肺癌和结肠癌的易感性增加。我们推测：（a）GSTM1基因缺失的个体患卵巢癌的风险也可能增加；（b）GSTM1基因型会影响化疗反应。使用针对GSTM1基因和内部对照谷胱甘肽S-转移酶μ-4（GSTM4）的三引物PCR反应，对146例浸润性上皮性卵巢癌患者进行基因分型。产物在琼脂糖凝胶上进行分析。无卵巢癌、乳腺癌或结肠癌家族史的健康个体作为非匹配对照（n = 80）。结果显示，卵巢癌的诊断年龄、组织学类型和分期均与GSTM1基因型无关。卵巢癌队列中GSTM1基因缺失基因型的频率与对照人群相似，分别为51%和58%，P > 0.05。同样，晚期卵巢癌患者的中位生存期与GSTM1基因型无关。我们得出结论，GSTM1基因缺失基因型不会增加卵巢癌风险。这些发现表明，GSTM1在解毒影响卵巢癌发生的环境因素方面不起重要作用，在卵巢癌对化疗的耐药性方面也不起重要作用。
