Ingkaninan K, Hazekamp A, de Best C M, Irth H, Tjaden U R, van der Heijden R, van der Greef J, Verpoorte R
Division of Pharmacognosy, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands.
J Nat Prod. 2000 Jun;63(6):803-6. doi: 10.1021/np9905719.
An HPLC with on-line coupled UV/MS-biochemical detection method for acetylcholinesterase (AChE) inhibitors in natural sources has been developed. The potential of this method is shown by the isolation of a new AChE inhibitor from the alcoholic extract of Narcissus 'Sir Winston Churchill'. Combining a prefractionation technique using centrifugal partition chromatography with the on-line HPLC-UV/MS-biochemical detection resulted in the isolation of the active compound that was identified as ungiminorine. This alkaloid shows a mild inhibitory effect on AChE.
已开发出一种用于天然来源中乙酰胆碱酯酶（AChE）抑制剂的高效液相色谱（HPLC）与在线联用紫外/质谱 - 生化检测方法。从水仙‘温斯顿·丘吉尔爵士’的乙醇提取物中分离出一种新型AChE抑制剂，证明了该方法的潜力。将使用离心分配色谱的预分离技术与在线HPLC - 紫外/质谱 - 生化检测相结合，分离出了被鉴定为去甲滇乌碱的活性化合物。这种生物碱对AChE显示出轻微的抑制作用。
