Housley G D, Thorne P R
Department of Physiology, University of Auckland, Private Bag 92019, Auckland, New Zealand.
J Auton Nerv Syst. 2000 Jul 3;81(1-3):139-45. doi: 10.1016/s0165-1838(00)00116-8.
Investigation of the multiple roles of extracellular nucleotides in the cochlea has developed from analysis of ATP-activated conductances in single sensory hair cells. Molecular probes such as radiolabelled ATP analogues and radiolabelled mRNA for ATP-gated ion channel subunits (P2X receptors) rapidly revealed the extensive nature of ATP signalling in this sensory organ. This has provided a foundation for physiological investigations which put extracellular nucleotides at the centre of homeostatic regulation of the driving force for sound transduction, modulation of mechanical tuning, control of cochlear blood flow and auditory neurotransmission. The purinergic signal transduction pathways associated with these processes have several novel features of significance to the broader field of purinergic neuroscience. In turn, these studies have benefited from the recent experimental advances in the field of purinergic signalling, a significant component of which is associated with the work of Professor Geoffrey Burnstock.
对细胞外核苷酸在耳蜗中多种作用的研究，是从对单个感觉毛细胞中ATP激活电导的分析发展而来的。诸如放射性标记的ATP类似物和ATP门控离子通道亚基（P2X受体）的放射性标记mRNA等分子探针，迅速揭示了该感觉器官中ATP信号传导的广泛性质。这为生理学研究奠定了基础，这些研究将细胞外核苷酸置于声音转导驱动力的稳态调节、机械调谐的调节、耳蜗血流的控制和听觉神经传递的中心位置。与这些过程相关的嘌呤能信号转导途径具有几个对嘌呤能神经科学更广泛领域具有重要意义的新特征。反过来，这些研究受益于嘌呤能信号传导领域最近的实验进展，其中一个重要组成部分与杰弗里·伯恩斯托克教授的工作有关。
