Levine M, Movafagh B F, Schwartzott D L
Department of Biochemistry and Molecular Biology, University of Oklahoma at Oklahoma City 73190, USA.
Oral Microbiol Immunol. 1987 Jun;2(2):88-91. doi: 10.1111/j.1399-302x.1987.tb00296.x.
The effects of heat labile, high molecular weight water-soluble toxins from bacterial plaque on HL60 promyelocytic cells were examined. On gel filtration, four inhibitors of HL60 cell growth and two inhibitors of HeLa cell growth (PT1, PT2) were detected. The first and third HL60 cell inhibitors corresponded to the two HeLa cell inhibitors. The last eluted HL60 cell inhibitor (plaque leukotoxin, PL) did not inhibit HeLa cell growth. Anti-PT2 antibodies reduced the activity of enriched PT2 by 20-50%, but all other antisera tested exhibited no effect. Anti-PL antibodies detected antigens from Actinobacillus actinomycetemcomitans, although anti-A. actinomycetemcomitans and anti-Capnocytophaga sputigena antibodies did not react with plaque extract. These findings suggest that the plaque toxins examined in this study were probably not derived from these two bacteria.
研究了来自牙菌斑的热不稳定、高分子量水溶性毒素对HL60早幼粒细胞的影响。在凝胶过滤中，检测到四种HL60细胞生长抑制剂和两种HeLa细胞生长抑制剂（PT1、PT2）。第一种和第三种HL60细胞抑制剂与两种HeLa细胞抑制剂相对应。最后洗脱的HL60细胞抑制剂（菌斑白细胞毒素，PL）不抑制HeLa细胞生长。抗PT2抗体使富集的PT2活性降低20 - 50%，但所检测的所有其他抗血清均无作用。抗PL抗体检测到来自伴放线放线杆菌的抗原，尽管抗伴放线放线杆菌抗体和抗牙龈二氧化碳嗜纤维菌抗体与菌斑提取物不发生反应。这些发现表明，本研究中检测的菌斑毒素可能并非源自这两种细菌。
